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Fc gamma RIIB/CD32b Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-14905

Novus Biologicals, part of Bio-Techne
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NBP2-14905

Key Product Details

Validated by

Orthogonal Validation

Species Reactivity

Validated:

Human

Predicted:

Rhesus Macaque (92%). Backed by our 100% Guarantee.

Applications

Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Product Specifications

Immunogen

Carrier-protein conjugated synthetic peptide encompassing a sequence within the C-terminus region of human Fc gamma RIIB/CD32b. The exact sequence is proprietary.

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

34 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for Fc gamma RIIB/CD32b Antibody

Western Blot: Fc gamma RIIB/CD32b Antibody [NBP2-14905]

Western Blot: Fc gamma RIIB/CD32b Antibody [NBP2-14905]

Western Blot: Fc gamma RIIB/CD32b Antibody [NBP2-14905] - Image submitted by a verified customer review.
Immunocytochemistry/ Immunofluorescence: Fc gamma RIIB/CD32b Antibody [NBP2-14905]

Immunocytochemistry/ Immunofluorescence: Fc gamma RIIB/CD32b Antibody [NBP2-14905]

Immunocytochemistry/Immunofluorescence: Fc gamma RIIB/CD32b Antibody [NBP2-14905] - Analysis of methanol-fixed A431, using antibody at 1:500 dilution.
Fc gamma RIIB/CD32b Antibody

Western Blot: Fc gamma RIIB/CD32b Antibody [NBP2-14905] -

Western Blot: Fc gamma RIIB/CD32b Antibody [NBP2-14905] - Various whole cell extracts (30 ug) were separated by 12% SDS-PAGE, and the membrane was blotted with CD32B antibody [C2C3], C-term diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody. Corresponding RNA expression data for the same cell lines are based on Human Protein Atlas program.

Applications for Fc gamma RIIB/CD32b Antibody

Application
Recommended Usage

Flow Cytometry

Assay-dependent dilution.

Immunocytochemistry/ Immunofluorescence

1:100 - 1:1000

Western Blot

1:500 - 1:3000
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Reviewed Applications

Read 1 review rated 5 using NBP2-14905 in the following applications:

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Formulation

PBS (pH 7), 20% Glycerol

Preservative

0.01% Thimerosal

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: Fc gamma RIIB/CD32b

The protein encoded by this gene is a low affinity receptor for the Fc region of immunoglobulin gamma complexes. The encoded protein is involved in the phagocytosis of immune complexes and in the regulation of antibody production by B-cells. Variations in this gene may increase susceptibilty to systemic lupus erythematosus (SLE). Several transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq]

Long Name

Fc gamma Receptor II

Alternate Names

CD32b, FCGR2B, FcgRIIB, FCRIIB

Gene Symbol

FCGR2B

UniProt

Additional Fc gamma RIIB/CD32b Products

Product Documents for Fc gamma RIIB/CD32b Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for Fc gamma RIIB/CD32b Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause reproductive toxicity with developmental effects.  For more information go to www.P65Warnings.ca.gov.