FCAR/CD89 Antibody (14A8 (14.1)) - Chimeric - Azide and BSA Free
Novus Biologicals, part of Bio-Techne | Catalog # NBP3-20161
Recombinant Monoclonal Antibody
Conjugate
Catalog #
Key Product Details
Species Reactivity
Human
Applications
ELISA, Flow Cytometry, Immunoprecipitation
Label
Unconjugated
Antibody Source
Recombinant Monoclonal Rabbit IgG Kappa Clone # 14A8 (14.1)
Format
Azide and BSA Free
Concentration
1 mg/ml
Product Specifications
Immunogen
The original antibody was generated by immunizing HCO12 trangene containing mice with recombinant soluble CD89 antigen.
Epitope
This antibody binds CD89 in the extracellular domain 2, outside the IgA-binding site.
Clonality
Monoclonal
Host
Rabbit
Isotype
IgG Kappa
Scientific Data Images for FCAR/CD89 Antibody (14A8 (14.1)) - Chimeric - Azide and BSA Free
Flow Cytometry: FCAR/CD89 Antibody (14A8 (14.1)) - Chimeric - Azide and BSA Free [NBP3-20161] -
Flow Cytometry: FCAR/CD89 Antibody (14A8 (14.1)) - Chimeric - Azide and BSA Free [NBP3-20161] - Paraformaldehyde fixed human peripheral blood monocytes permeabilized with 0.5% Triton were stained with anti-unknown specificity antibody (isotype control, black line) or the rabbit IgG version of 14A8 (14.1) (NBP3-20161, blue line) at a dilution of 1:100 for 1h at RT. After washing, the bound antibody was detected using a goat anti-rabbit IgG AlexaFluor(R) 488 antibody at a dilution of 1:1000 and cells analyzed using a FACSCanto flow-cytometer.Applications for FCAR/CD89 Antibody (14A8 (14.1)) - Chimeric - Azide and BSA Free
Application
Recommended Usage
ELISA
Optimal dilutions of this antibody should be experimentally determined.
Flow Cytometry
Optimal dilutions of this antibody should be experimentally determined.
Immunoprecipitation
Optimal dilutions of this antibody should be experimentally determined.
Application Notes
This chimeric rabbit antibody was made using the variable domain sequences of the original Mouse IgG1 format, for improved compatibility with existing reagents, assays and techniques. It binds human CD89 with a dissociation constant of Kd= 6.8 X10-10M.
Formulation, Preparation, and Storage
Purification
Protein A purified
Formulation
PBS
Format
Azide and BSA Free
Preservative
0.02% Proclin 300
Concentration
1 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C for up to 3 months. For longer storage, aliquot and store at -20C.
Background: FCAR/CD89
Long Name
IgA Fc Receptor
Alternate Names
CD89
Gene Symbol
FCAR
Additional FCAR/CD89 Products
Product Documents for FCAR/CD89 Antibody (14A8 (14.1)) - Chimeric - Azide and BSA Free
Product Specific Notices for FCAR/CD89 Antibody (14A8 (14.1)) - Chimeric - Azide and BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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