FCRN/FCGRT Antibody

Knockout Validated: FCRN/FCGRT Antibody [NBP1-89128]

Knockout Validated: FCRN/FCGRT Antibody [NBP1-89128] - Western blot shows lysates of 293 human embryonic kidney parental cell line and FCGRT knockout (KO) 293 cell line. PVDF membrane was probed with 1:500 of Rabbit Anti-Human FCGRT Polyclonal Antibody (Catalog # NBP1-89128) followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog #HAF008). Specific band was detected for FCGRT at approximately 40 kDa (as indicated) in the parental 293 cell line, but is not detectable in the knockout 293 cell line. This experiment was conducted under reducing conditions.

Western Blot: FCRN/FCGRT Antibody [NBP1-89128]

Western Blot: FCRN/FCGRT Antibody [NBP1-89128] - Analysis in human cell lines Caco-2 and HeLa using anti-FCGRT antibody. Corresponding FCGRT RNA-seq data are presented for the same cell lines. Loading control: anti-GAPDH.

Western Blot: FCRN/FCGRT Antibody [NBP1-89128]

Western Blot: FCRN/FCGRT Antibody [NBP1-89128] - Human lung epithelial A549 cells and primary bronchial epithelial cells (hAECB) lack FcgammaR (CD16, CD64, and CD32) and FcalphaR (CD89) cell surface expression as analyzed by flow cytometry (human THP1 cells as positive control). The neonatal FcR (FcRn) is expressed in both A549 cells & human THP1 cells as determined by Western blotting. Data are representative of at least two independent experiments. Absolute bacterial counts (CFUs) in human THP1 macrophages 1 h after infection with MTB preincubated with the LAM-reactive IgA1 (left) or IgG1 (right) antibody TB24PB037 or the respective isotype control antibody as indicated. Mean and SEM are indicated. Data are representative of three independent experiments. Image collected and cropped by CiteAb from the following publication (https://embomolmed.embopress.org/lookup/doi/10.15252/emmm.201606330), licensed under a CC-BY license.