FDFT1 Antibody (10H12)
Novus Biologicals, part of Bio-Techne | Catalog # NBP3-15199
Recombinant Monoclonal Antibody
Key Product Details
Species Reactivity
Validated:
Human
Applications
ELISA, Immunohistochemistry, Western Blot
Label
Unconjugated
Antibody Source
Recombinant Monoclonal Rabbit IgG Clone # 10H12
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Product Summary for FDFT1 Antibody (10H12)
Immunogen
A synthesized peptide derived from human FDFT1. UniProt ID (P37268).
Clonality
Monoclonal
Host
Rabbit
Isotype
IgG
Scientific Data Images for FDFT1 Antibody (10H12)
Western Blot: FDFT1 Antibody (10H12) [NBP3-15199]
Western Blot: FDFT1 Antibody (10H12) [NBP3-15199] - Positive WB detected in: THP-1 whole cell lysate, K562 whole cell lysate. All lanes: FDFT1 antibody at 1:1000. Secondary: Goat polyclonal to rabbit IgG at 1/50000 dilution. Predicted band size: 49, 41, 39, 36, 44 KDa. Observed band size: 50 KDa.Immunohistochemistry: FDFT1 Antibody (10H12) [NBP3-15199]
Immunohistochemistry: FDFT1 Antibody (10H12) [NBP3-15199] - IHC image of FDFT1 antibody diluted at 1:100 and staining in paraffin-embedded human lung cancer. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30 minutes at RT. Then primary antibody (1% BSA) was incubated at 4C overnight. The primary is detected by a Goat anti-rabbit IgG polymer labeled by HRP and visualized using 0.05% DAB.Immunohistochemistry: FDFT1 Antibody (10H12) [NBP3-15199]
Immunohistochemistry: FDFT1 Antibody (10H12) [NBP3-15199] - IHC image of FDFT1 antibody diluted at 1:100 and staining in paraffin-embedded human testis tissue. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30 minutes at RT. Then primary antibody (1% BSA) was incubated at 4C overnight. The primary is detected by a Goat anti-rabbit IgG polymer labeled by HRP and visualized using 0.05% DAB.Applications for FDFT1 Antibody (10H12)
Application
Recommended Usage
Immunohistochemistry
1:50-1:200
Western Blot
1:500-1:5000
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Affinity purified
Formulation
PBS (pH 7.4), 150mM NaCl, and 50% glycerol.
Preservative
0.02% Sodium Azide
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at -20 to -80C. Avoid freeze-thaw cycles.
Background: FDFT1
Long Name
Squalene synthase
Alternate Names
EC 2.5.1.21, SQS, SS
Gene Symbol
FDFT1
Additional FDFT1 Products
Product Documents for FDFT1 Antibody (10H12)
Product Specific Notices for FDFT1 Antibody (10H12)
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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