Feline IL-5 Antibody
R&D Systems, part of Bio-Techne | Catalog # MAB1795
Key Product Details
Species Reactivity
Feline
Applications
ELISA Capture (Matched Antibody Pair), Neutralization
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG2A Clone # 604312
Product Specifications
Immunogen
Mouse myeloma cell line NS0-derived recombinant feline IL-5
Ile20-Ser134
Accession # O77515
Ile20-Ser134
Accession # O77515
Specificity
Detects feline IL-5 in ELISAs. In sandwich immunoassays, no cross-reactivity with recombinant feline GM-CSF, recombinant equine, canine, mouse, human, or porcine IL-5 is observed.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG2A
Endotoxin Level
<0.10 EU per 1 μg of the antibody by the LAL method.
Scientific Data Images for Feline IL-5 Antibody
Cell Proliferation Induced by IL‑5 and Neutralization by Feline IL‑5 Antibody.
Recombinant Feline IL-5 (Catalog # 1795-FL) stimulates proliferation in the TF-1 human erythroleukemic cell line in a dose-dependent manner (orange line). Proliferation elicited by Recombinant Feline IL-5 (30 ng/mL) is neutralized (green line) by increasing concentrations of Mouse Anti-Feline IL-5 Monoclonal Antibody (Catalog # MAB1795). The ND50 is typically 0.1-0.5 µg/mL.Applications for Feline IL-5 Antibody
Application
Recommended Usage
Neutralization
Measured by its ability to neutralize IL‑5-induced proliferation in the TF‑1 human erythroleukemic cell line. Kitamura, T. et al. (1989) J. Cell Physiol. 140:323. The Neutralization Dose (ND50) is typically 0.1-0.5 µg/mL in the presence of 30 ng/mL Recombinant Feline IL‑5.
Feline IL-5 Sandwich Immunoassay
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Protein A or G purified from hybridoma culture supernatant
Reconstitution
Sterile PBS to a final concentration of 0.5 mg/mL. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: IL-5
Interleukin-5 (IL-5) is a 40 kDa secreted, heparin-binding, disulfide-linked homodimeric glycoprotein that belongs to the alpha-helical group of cytokines. It promotes the proliferation, differentiation and activation of eosinophils. The 115 amino acid (aa) mature feline IL-5 shares 70% and 59% aa sequence identity with human and mouse IL-5, respectively. The IL-5 receptor is a complex of an IL-5-specific ligand-binding alpha subunit with a signal transducing common beta subunit ( betac) that is shared with receptor complexes for IL-3 and GM-CSF.
Long Name
Interleukin 5
Alternate Names
BCDF mu, BCGFII, EDF, Eo-CSF, IL5, TRF
Entrez Gene IDs
Gene Symbol
IL5
UniProt
Additional IL-5 Products
Product Documents for Feline IL-5 Antibody
Product Specific Notices for Feline IL-5 Antibody
For research use only
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