FEN-1 Antibody (4E7)
Novus Biologicals, part of Bio-Techne | Catalog # NB100-150
Key Product Details
Species Reactivity
Validated:
Human, Mouse
Cited:
Human
Applications
Validated:
Chromatin Immunoprecipitation (ChIP), Immunocytochemistry/ Immunofluorescence, Immunoprecipitation, Western Blot
Cited:
Block/Neutralize, Simple Western, Western Blot
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG1 Clone # 4E7
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Product Specifications
Immunogen
Recombinant human FEN-1 protein encoding amino acids 1-380 purified E. coli.
Reactivity Notes
Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any questions.
Localization
Nuclear
Specificity
This is specific for FEN-1.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG1
Theoretical MW
42 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Scientific Data Images for FEN-1 Antibody (4E7)
![Western Blot: FEN-1 Antibody (4E7) [NB100-150]](https://resources.bio-techne.com/images/products/FEN-1-Antibody-4E7-Western-Blot-NB100-150-img0006.jpg "Western Blot: FEN-1 Antibody (4E7) [NB100-150]")
Western Blot: FEN-1 Antibody (4E7) [NB100-150]
Western Blot: FEN-1 Antibody (4E7) [NB100-150] - Whole cell extract (30 ug) was separated by 12% SDS-PAGE, and the membrane was blotted with FEN1 antibody diluted at 1:500.![Immunocytochemistry/ Immunofluorescence: FEN-1 Antibody (4E7) [NB100-150]](https://resources.bio-techne.com/images/products/FEN-1-Antibody-4E7-Immunocytochemistry-Immunofluorescence-NB100-150-img0004.jpg "Immunocytochemistry/ Immunofluorescence: FEN-1 Antibody (4E7) [NB100-150]")
Immunocytochemistry/ Immunofluorescence: FEN-1 Antibody (4E7) [NB100-150]
Immunocytochemistry/Immunofluorescence: FEN-1 Antibody (4E7) [NB100-150] - Analysis of paraformaldehyde-fixed MCF7, using antibody at 1:200 dilution.Applications for FEN-1 Antibody (4E7)
Application
Recommended Usage
Western Blot
1:500-1:3000
Application Notes
Chip, ICC/IF,IP- Assay dependent
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Protein G purified
Formulation
PBS
Preservative
No Preservative
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Background: FEN-1
Long Name
Flap Endonuclease 1
Alternate Names
FEN1
Entrez Gene IDs
2237 (Human)
Gene Symbol
FEN1
UniProt
Additional FEN-1 Products
Product Documents for FEN-1 Antibody (4E7)
Product Specific Notices for FEN-1 Antibody (4E7)
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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