Key Product Details

Validated by

Biological Validation

Species Reactivity

Validated:

Human, Mouse

Cited:

Human

Predicted:

Bovine (100%), Rat (100%), Sheep (100%). Backed by our 100% Guarantee.

Applications

Validated:

Immunoprecipitation, Western Blot

Cited:

Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

1.0 mg/ml

View all FEN-1 Antibodies »

Product Specifications

Immunogen

The immunogen recognized by this antibody maps to a region between residues 75 and 125 of human Flap Structure Endonuclease 1 using the numbering given in entry NP_004102.1 (GeneID 2237).

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

43 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for FEN-1 Antibody

Western Blot: FEN-1 Antibody [NB100-320]

Western Blot: FEN-1 Antibody [NB100-320]

Western Blot: FEN-1 Antibody [NB100-320] - Detection of Human and Mouse Fen1 by Western Blot. Samples: Whole cell lysate (50 ug) from HeLa, 293T, and mouse NIH3T3 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-Fen1 antibody NB100-320 used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.
Immunoprecipitation: FEN-1 Antibody [NB100-320]

Immunoprecipitation: FEN-1 Antibody [NB100-320]

Immunoprecipitation: FEN-1 Antibody [NB100-320] - Whole cell lysate from HeLa cells (1 mg loaded), affinity purified rabbit antiFen1 antibody used for IP at 3 ug/mg lysate. Fen1 was also immunoprecipitated by rabbit anti-Fen1 antibody NB100-321, which recognizes a downstream epitope.

Applications for FEN-1 Antibody

Application
Recommended Usage

Immunoprecipitation

2 - 10 ug/mg lysate

Western Blot

1:2000 - 1:10000
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

Tris-Citrate/Phosphate (pH 7.0 - 8.0)

Preservative

0.09% Sodium Azide

Concentration

1.0 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C. Do not freeze.

Background: FEN-1

FEN-1 (Flap Endonuclease and Five prime Exonuclease) is a 42 kDa protein that functions as an endonuclease that cleaves branched DNA structures containing single-stranded 5' flaps or as an exonuclease on DNA nicks and gaps. FEN-1 endonuclease and exonuclease activities are stimulated by an association with proliferating cell nuclear antigen (PCNA) in the nucleus. FEN-1 is believed to have important roles in DNA replication and repair and is required in cell-free DNA replication systems.

Long Name

Flap Endonuclease 1

Alternate Names

FEN1

Entrez Gene IDs

2237 (Human)

Gene Symbol

FEN1

UniProt

P39748 (Human)

Additional FEN-1 Products

Product Documents for FEN-1 Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Download SDS

Product Specific Notices for FEN-1 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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