Ferritin Antibody (12H1)
Novus Biologicals, part of Bio-Techne | Catalog # NBP3-26568
Recombinant monoclonal antibody expressed in HEK293F cells
Key Product Details
Species Reactivity
Validated:
Human, Mouse
Applications
ELISA, Flow Cytometry, Immunohistochemistry, Western Blot
Label
Unconjugated
Antibody Source
Recombinant Monoclonal Mouse IgG Clone # 12H1
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Product Summary for Ferritin Antibody (12H1)
Immunogen
Recombinant Human Ferritin protein [UniProt P02794]
Clonality
Monoclonal
Host
Mouse
Isotype
IgG
Scientific Data Images for Ferritin Antibody (12H1)
Immunohistochemistry: Ferritin Antibody (12H1) [NBP3-26568] -
Immunohistochemistry: Ferritin Antibody (12H1) [NBP3-26568] - Image of Ferritin Antibody (12H1) diluted at 1:50 and staining in paraffin-embedded human liver tissue performed. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-Mouse IgG labeled by HRP and visualized using 0.05% DAB.
Immunohistochemistry: Ferritin Antibody (12H1) [NBP3-26568] -
Immunohistochemistry: Ferritin Antibody (12H1) [NBP3-26568] - Image of Ferritin Antibody (12H1) diluted at 1:50 and staining in paraffin-embedded human liver tissue performed. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-Mouse IgG labeled by HRP and visualized using 0.05% DAB.
Western Blot: Ferritin Antibody (12H1) [NBP3-26568] -
Western Blot: Ferritin Antibody (12H1) [NBP3-26568] - Positive Western Blot detected in: U251 whole cell lysate, JK whole cell lysate.All lanes: Ferritin Antibody at 1: 500
Secondary: Goat polyclonal to mouse IgG at 1/50000 dilution.
Predicted band size: 22 kDa
Observed band size: 22 kDa
Applications for Ferritin Antibody (12H1)
Application
Recommended Usage
Flow Cytometry
1:50-1:200
Immunohistochemistry
1:50-1:200
Western Blot
1:500-1:2000
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Affinity purified
Formulation
50% Glycerol, 0.01M PBS, PH 7.4
Preservative
0.03% Proclin 300
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at -20 to -70C. Avoid freeze-thaw cycles.
Background: Ferritin
Alternate Names
apoferritin, Cell proliferation-inducing gene 15 protein, Ferritin H subunit, ferritin heavy chain, ferritin, heavy polypeptide 1, FHC, FTHEC 1.16.3.1, FTHL6MGC104426, PIG15, placenta immunoregulatory factor, PLIF, proliferation-inducing protein 15
Gene Symbol
FTH1
Additional Ferritin Products
Product Specific Notices for Ferritin Antibody (12H1)
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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