Ferritin Antibody
Novus Biologicals, part of Bio-Techne | Catalog # NB600-920
Key Product Details
Species Reactivity
Validated:
Human, Mouse
Cited:
Human, Mouse
Applications
Validated:
ELISA, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot
Cited:
Immunoprecipitation, Western Blot
Label
Unconjugated
Antibody Source
Polyclonal Rabbit IgG
Concentration
LYOPH mg/ml
Product Specifications
Immunogen
Ferritin [Human Spleen] (Uniprot: P02794)
Specificity
Cross reactivity against Ferritin Heavy Chain from other tissues and species may occur but have not been specifically determined.
Clonality
Polyclonal
Host
Rabbit
Isotype
IgG
Description
This antibody is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Rabbit Serum as well as purified and partially purified Ferritin [Human Spleen]
Store vial at 4C prior to restoration. For extended storage aliquot contents and freeze at -20C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4C as an undiluted liquid. Dilute only prior to immediate use.
Store vial at 4C prior to restoration. For extended storage aliquot contents and freeze at -20C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4C as an undiluted liquid. Dilute only prior to immediate use.
Scientific Data Images for Ferritin Antibody
![Western Blot: Ferritin Heavy Chain Antibody [NB600-920]](https://resources.bio-techne.com/images/products/Ferritin-Antibody-Western-Blot-NB600-920-img0004.jpg "Western Blot: Ferritin Antibody [NB600-920]")
Western Blot: Ferritin Antibody [NB600-920]
Western Blot: Ferritin Antibody [NB600-920] - Lane 1: Ferritin. Lane 2: None. Load: 50 ng per lane. Primary antibody: Ferritin primary antibody at 1:1,000 overnight at 4C. Secondary antibody: Peroxidase rabbit secondary antibody at 1:40,000 for 30 min at RT. Blocking: incubated with blocking buffer for 30 min at RT. Predicted/Observed size: 20 kDa, 20 kDa for Ferritin. Other band(s): None.Applications for Ferritin Antibody
Application
Recommended Usage
ELISA
1:5000-1:20000
Immunohistochemistry
1:200-1:1000
Immunohistochemistry-Paraffin
1:200-1:1000
Western Blot
1:5000-1:2000
Application Notes
This product has been tested in ELISA and western blot and is suitable for use in immunohistochemistry. Specific conditions for reactivity should be optimized by the end user.
Formulation, Preparation, and Storage
Purification
Multi-step
Reconstitution
Reconstitute with 100 ul deionized water (or equivalent)
Formulation
Lyophilized from 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Preservative
0.01% Sodium Azide
Concentration
LYOPH mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store lyophilized antibody at 4C. Aliquot reconstituted liquid and store at -20C. Avoid freeze-thaw cycles.
Background: Ferritin
Alternate Names
apoferritin, Cell proliferation-inducing gene 15 protein, Ferritin H subunit, ferritin heavy chain, ferritin, heavy polypeptide 1, FHC, FTHEC 1.16.3.1, FTHL6MGC104426, PIG15, placenta immunoregulatory factor, PLIF, proliferation-inducing protein 15
Entrez Gene IDs
2495 (Human)
Gene Symbol
FTH1
UniProt
Additional Ferritin Products
Product Documents for Ferritin Antibody
Product Specific Notices for Ferritin Antibody
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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