Ferritin Light Chain Antibody (X1)

Novus Biologicals, part of Bio-Techne | Catalog # H00002512-M16

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Product Datasheet / COA / SDS

Product Specifications
Scientific Data
Applications
Preparation & Storage
Background
Product Documents
Specific Notices

Key Product Details

Species Reactivity

Human

Applications

ELISA, Immunocytochemistry/ Immunofluorescence, Immunoprecipitation, Knockdown Validated, Sandwich ELISA, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG₁ kappa Clone # X1

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

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Product Specifications

Immunogen

FTL (AAH04245, 1 a.a. ~ 175 a.a) full-length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. MSSQIRQNYSTDVEAAVNSLVNLYLQASYTYLSLGFYFDRDDVALEGVSHFFRELAEEKREGYERLLKMQNQRGGRALFQDIKKPAEDEWGKTPDAMKAAMALEKKLNQALLDLHALGSARTDPHLCDFLETHFLDEEVKLIKKMGDHLTNLHRLGGPEAGLGEYLFERLTLKHD

Clonality

Monoclonal

Host

Mouse

Isotype

IgG₁ kappa

Description

Quality control test: Antibody Reactive Against Recombinant Protein.

Scientific Data Images for Ferritin Light Chain Antibody (X1)

Western Blot: Ferritin Light Chain Antibody (X1) [H00002512-M16]

Western Blot: Ferritin Light Chain Antibody (X1) [H00002512-M16] - Analysis of FTL expression in transfected 293T cell line by FTL monoclonal antibody (M16), clone X1. Lane 1: FTL transfected lysatE (20 KDa). Lane 2: Non-transfected lysate.

Immunocytochemistry/ Immunofluorescence: Ferritin Light Chain Antibody (X1) [H00002512-M16]

Immunocytochemistry/Immunofluorescence: Ferritin Light Chain Antibody (X1) [H00002512-M16] - Analysis of monoclonal antibody to FTL on HeLa cell. Antibody concentration 10 ug/ml

Western Blot: Ferritin Light Chain Antibody (X1) [H00002512-M16]

Western Blot: Ferritin Light Chain Antibody (X1) [H00002512-M16] - Analysis of FTL expression in K-562 (Cat # L009V1).

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Applications for Ferritin Light Chain Antibody (X1)

Application

Recommended Usage

ELISA

Optimal dilutions of this antibody should be experimentally determined.

Immunocytochemistry/ Immunofluorescence

Optimal dilutions of this antibody should be experimentally determined.

Immunoprecipitation

Optimal dilutions of this antibody should be experimentally determined.

Knockdown Validated

Optimal dilutions of this antibody should be experimentally determined.

Sandwich ELISA

Optimal dilutions of this antibody should be experimentally determined.

Western Blot

Optimal dilutions of this antibody should be experimentally determined.

Application Notes

It has been used for ELISA, WB, IP and IF.

Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

IgG purified

Formulation

In 1x PBS, pH 7.4

Preservative

No Preservative

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: Ferritin Light Chain

This gene encodes the light subunit of the ferritin protein. Ferritin is the major intracellular iron storage protein in prokaryotes and eukaryotes. It is composed of 24 subunits of the heavy and light ferritin chains. Variation in ferritin subunit composition may affect the rates of iron uptake and release in different tissues. A major function of ferritin is the storage of iron in a soluble and nontoxic state. Defects in this light chain ferritin gene are associated with several neurodegenerative diseases and hyperferritinemia-cataract syndrome. This gene has multiple pseudogenes. [provided by RefSeq, Jul 2008]

Alternate Names

Ferritin L subunit, ferritin L-chain, ferritin light chain, ferritin light polypeptide-like 3, ferritin, light polypeptide, MGC71996, NBIA3

Gene Symbol

FTL

UniProt

AAH04245 (Human)

Additional Ferritin Light Chain Products

Product Documents for Ferritin Light Chain Antibody (X1)

Product Datasheets

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COA

SDS

Product Specific Notices for Ferritin Light Chain Antibody (X1)

This product is produced by and distributed for Abnova, a company based in Taiwan.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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