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Ferritin Light Chain Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-06986

Novus Biologicals, part of Bio-Techne
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NBP1-06986

Key Product Details

Species Reactivity

Validated:

Human, Mouse, Rat

Cited:

Human, Mouse, Rat

Predicted:

Bovine (100%), Canine (100%). Backed by our 100% Guarantee.

Applications

Validated:

Immunohistochemistry, Peptide ELISA, Western Blot

Cited:

Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Goat IgG

Concentration

0.5 mg/ml

Product Specifications

Immunogen

Peptide with sequence C-GEYLFERLTLKHD corresponding to C-Terminus according to NP_000137.2.

Reactivity Notes

Rat reactivity reported in the literature (PMID: 21922429)

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Scientific Data Images for Ferritin Light Chain Antibody

Western Blot: Ferritin Light Chain Antibody [NBP1-06986]

Western Blot: Ferritin Light Chain Antibody [NBP1-06986]

Western Blot: Ferritin Light Chain Antibody [NBP1-06986] - Human Brain (Cerebellum) (A), Liver (B), Human Placenta (C) 22k Da in MBR (D), RBR (E) lysate (35 ug protein in RIPA buffer). Primary antibody at 0.3 ug/mL, 1 hr incubation. Detected by chemiluminescence.
Immunohistochemistry: Ferritin Light Chain Antibody [NBP1-06986]

Immunohistochemistry: Ferritin Light Chain Antibody [NBP1-06986]

Immunohistochemistry: Ferritin Light Chain Antibody [NBP1-06986] - Negative Control showing staining of paraffin embedded Human Spleen, with no primary antibody.
Immunohistochemistry: Ferritin Light Chain Antibody [NBP1-06986]

Immunohistochemistry: Ferritin Light Chain Antibody [NBP1-06986]

Immunohistochemistry: Ferritin Light Chain Antibody [NBP1-06986] - (4ug/ml) staining of paraffin embedded Human Spleen. Heat induced antigen retrieval with citrate buffer pH 6, HRP-staining.

Applications for Ferritin Light Chain Antibody

Application
Recommended Usage

Immunohistochemistry

4 - 6 ug/ml

Peptide ELISA

Detection limit 1:32000

Western Blot

0.3 - 1.0 ug/mL
Application Notes
Western blot: Approx. 20 kDa band observed in Human Brain (Cerebellum), Liver and Placenta lysates and approx. 22 kDa band obseerved in Mouse and Rat Brain lysates (calculated MW of 20.0 kDa according to NP_000137.2).

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA

Preservative

0.02% Sodium Azide

Concentration

0.5 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: Ferritin Light Chain

This gene encodes the light subunit of the ferritin protein. Ferritin is the major intracellular iron storage protein in prokaryotes and eukaryotes. It is composed of 24 subunits of the heavy and light ferritin chains. Variation in ferritin subunit composition may affect the rates of iron uptake and release in different tissues. A major function of ferritin is the storage of iron in a soluble and nontoxic state. Defects in this light chain ferritin gene are associated with several neurodegenerative diseases and hyperferritinemia-cataract syndrome. This gene has multiple pseudogenes. [provided by RefSeq, Jul 2008]

Alternate Names

Ferritin L subunit, ferritin L-chain, ferritin light chain, ferritin light polypeptide-like 3, ferritin, light polypeptide, MGC71996, NBIA3

Entrez Gene IDs

2512 (Human); 14325 (Mouse); 29292 (Rat)

Gene Symbol

FTL

UniProt

Additional Ferritin Light Chain Products

Product Documents for Ferritin Light Chain Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for Ferritin Light Chain Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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