Fetuin A/AHSG Antibody

Western Blot: Fetuin A/AHSG Antibody [NBP1-02152]

Western Blot: Fetuin Antibody [NBP1-02152] - Analysis of fetuin in purified preparations and in human plasma. Lane 1 contains 250 ng of purified human fetuin. Lane 2 contains 5 l of a 1:50 dilution of human serum. Dilution of Anti-Human Fetuin antibody between 1:10,000 and 1:20,000 showed strong reactivity by western blot. In this blot the antibody was used at a 1:10,000 dilution incubated 1 h at room temperature in 1% BSA in TTBS. Detection occurred using a 1:5,000 dilution of IRDye800 conjugated Donkey anti-Goat IgG for 45 min at room temperature.

Fetuin A/AHSG Antibody

Western blot of Fetuin. Anti-Human Fetuin antibody, generated by immunization with mature protein, was tested by western blot against fetuin in purified preparations and in human plasma. Lane 1 contains 250 ng of purified human fetuin. Lane 2 contains 5 ul of a 1:50 dilution of human serum. Dilution of Anti-Human Fetuin antibody between 1:10,000 and 1:20,000 showed strong reactivity by western blot. In this blot the antibody was used at a 1:10,000 dilution incubated 1 h at room temperature in 1% BSA in TTBS. Detection occurred using a 1:5,000 dilution of IRDye(TM)800 conjugated Donkey anti-Goat IgG (code # ) for 45 min at room temperature. LICOR's Odyssey(R) Infrared Imaging System was used to scan and process the image. Other detection systems will yield similar results.