FGFR1 Antibody (M17D10) - BSA Free

Novus Biologicals, part of Bio-Techne | Catalog # NB100-2080

Novus Biologicals, part of Bio-Techne
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NB100-2080
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Key Product Details

Species Reactivity

Validated:

Human, Mouse

Cited:

Mouse

Applications

Validated:

CyTOF-ready, Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Immunoprecipitation, Western Blot

Cited:

Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # M17D10

Format

BSA Free

Concentration

1.0 mg/ml

View all FGFR1 Antibodies »

Product Summary for FGFR1 Antibody (M17D10) - BSA Free

Immunogen

Recombinant human ectodomain of FGFr1b expressed in E. coli from pro23 to his415; antigen contained NH2-terminal gly-ser-pro-gly-ile and COOH-terminal glu-phe sequences.

Epitope

Epitope is within the sequence pro179 to lys195 in Ig loop II.

Reactivity Notes

Mouse reactivity reported in scientific literature (PMID: 8575296). Not yet tested in other species.

Localization

Cell Membrane

Specificity

Reacts with both alpha and beta isoforms.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Scientific Data Images for FGFR1 Antibody (M17D10) - BSA Free

Immunocytochemistry/ Immunofluorescence: FGFR1 Antibody (M17D10) - BSA Free [NB100-2080]

Immunocytochemistry/ Immunofluorescence: FGFR1 Antibody (M17D10) - BSA Free [NB100-2080]

Immunocytochemistry/Immunofluorescence: FGFR1 Antibody (M17D10) [NB100-2080] - Ntera2 cells were fixed in 4% paraformaldehyde for 10 minutes and permeabilized in 0.05% Triton X-100 in PBS for 5 minutes. The cells were incubated with FGFR1 Antibody [M17D10] (NB100-2080) at 2 ug/ml overnight at 4C and detected with an anti-mouse Dylight 488 (Green) at a 1:1000 dilution for 60 minutes. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 40X objective.
Immunocytochemistry/ Immunofluorescence: FGFR1 Antibody (M17D10) - BSA Free [NB100-2080]

Immunocytochemistry/ Immunofluorescence: FGFR1 Antibody (M17D10) - BSA Free [NB100-2080]

Immunocytochemistry/Immunofluorescence: FGFR1 Antibody (M17D10) [NB100-2080] - Ntera2 cells were fixed in 4% paraformaldehyde for 10 minutes and permeabilized in 0.05% Triton X-100 in PBS for 5 minutes. The cells were incubated with FGFR1 Antibody [M17D10] conjugated to Alexa Fluor 488 (NB100-2080AF488) at 10 ug/ml for 1 hour at room temperature. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 40X objective.
Western Blot: FGFR1 Antibody (M17D10)BSA Free [NB100-2080]

Western Blot: FGFR1 Antibody (M17D10)BSA Free [NB100-2080]

Western Blot: FGF R1 Antibody (M17D10) [NB100-2080] - Total protein from MOLT-4 (lane 1) and Ntera-2 (lane 2) was separated on a 7.5% gel by SDS-PAGE. Protein was transferred to PVDF membrane, blocked and probed with 1 ug/mL of anti-FGF R1. FGF R1 protein was detected at ~150 kDa using an anti-mouse HRP secondary antibody.
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Applications for FGFR1 Antibody (M17D10) - BSA Free

Application
Recommended Usage

Flow Cytometry

1:500 - 1:1000

Immunocytochemistry/ Immunofluorescence

10 ug/mL

Immunohistochemistry

5 ug/mL

Immunohistochemistry-Paraffin

5 ug/mL

Immunoprecipitation

1:10 - 1:500

Western Blot

1 ug/mL
Application Notes
This antibody is CyTOF ready.
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Protein G purified

Formulation

PBS

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

1.0 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: FGFR1

FGF activity is mediated by a family of type I transmembrane tyrosine kinases, which undergo dimerization and autophosphorylation after ligand binding. Five distinct genes encode closely related FGF receptors, FGF R1 through 5. FGF Rs contain three Ig-like domains and a stretch of acidic residues between the first and second Ig-like domains. FGF R1, 2, 3, and -4 have a cytoplasmic split tyrosine-kinase domain, but FGF R5 does not. Multiple forms of FGF R1, 2, and 3 are generated by alternative splicing

Long Name

Fibroblast Growth Factor Receptor 1

Alternate Names

CD331, FGF R1, Flt-2

Entrez Gene IDs

2260 (Human)

Gene Symbol

FGFR1

UniProt

P11362

Additional FGFR1 Products

Product Documents for FGFR1 Antibody (M17D10) - BSA Free

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for FGFR1 Antibody (M17D10) - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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