FGFR1 [p Tyr653] Antibody - BSA Free

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-05382

Novus Biologicals, part of Bio-Techne
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Key Product Details

Validated by

Biological Validation

Species Reactivity

Validated:

Human, Mouse, Rat

Applications

Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

View all FGFR1 Antibodies »

Product Summary for FGFR1 [p Tyr653] Antibody - BSA Free

Immunogen

A synthetic phosphorylated peptide around Y653 of human FGFR1 (NP_075598.2). IDYYK

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for FGFR1 [p Tyr653] Antibody - BSA Free

Western Blot: FGFR1 [p Tyr653] AntibodyBSA Free [NBP3-05382]

Western Blot: FGFR1 [p Tyr653] AntibodyBSA Free [NBP3-05382]

Western Blot: FGFR1 [p Tyr653] Antibody [NBP3-05382] - Western blot analysis of extracts of 293T cells, using FGFR1 pAb (NBP3-05382) at 1:1000 dilution.293T cells were treated by EGF (25 ug/mL) at 37? for 30 minutes after serum-starvation overnight. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% BSA. Detection: ECL Basic Kit. Exposure time: 30s.

Applications for FGFR1 [p Tyr653] Antibody - BSA Free

Application
Recommended Usage

Western Blot

1:500-1:2000
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS with 50% glycerol, pH7.3.

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: FGFR1

FGF activity is mediated by a family of type I transmembrane tyrosine kinases, which undergo dimerization and autophosphorylation after ligand binding. Five distinct genes encode closely related FGF receptors, FGF R1 through 5. FGF Rs contain three Ig-like domains and a stretch of acidic residues between the first and second Ig-like domains. FGF R1, 2, 3, and -4 have a cytoplasmic split tyrosine-kinase domain, but FGF R5 does not. Multiple forms of FGF R1, 2, and 3 are generated by alternative splicing

Long Name

Fibroblast Growth Factor Receptor 1

Alternate Names

CD331, FGF R1, Flt-2

Gene Symbol

FGFR1

Additional FGFR1 Products

Product Documents for FGFR1 [p Tyr653] Antibody - BSA Free

Product Datasheets

Certificate of Analysis

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Product Specific Notices for FGFR1 [p Tyr653] Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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