FHL1 Antibody (OTI3G7) - Azide and BSA Free

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-70739

Novus Biologicals, part of Bio-Techne
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Key Product Details

Species Reactivity

Human, Mouse, Rat

Applications

CyTOF-ready, Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2B Clone # OTI3G7

Format

Azide and BSA Free

Concentration

LYOPH mg/ml

View all FHL1 Antibodies »

Product Specifications

Immunogen

Full length human recombinant protein of human FHL1 (NP_001440) produced in HEK293T cell.

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any questions.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2B

Theoretical MW

31.7 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for FHL1 Antibody (OTI3G7) - Azide and BSA Free

Western Blot: FHL1 Antibody (OTI3G7)Azide and BSA Free [NBP2-70739]

Western Blot: FHL1 Antibody (OTI3G7)Azide and BSA Free [NBP2-70739]

Western Blot: FHL1 Antibody (OTI3G7) - Azide and BSA Free [NBP2-70739] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY FHL1 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-FHL1.
Immunocytochemistry/ Immunofluorescence: FHL1 Antibody (OTI3G7) - Azide and BSA Free [NBP2-70739]

Immunocytochemistry/ Immunofluorescence: FHL1 Antibody (OTI3G7) - Azide and BSA Free [NBP2-70739]

Immunocytochemistry/Immunofluorescence: FHL1 Antibody (OTI3G7) - Azide and BSA Free [NBP2-70739] - Staining of COS7 cells transiently transfected by pCMV6-ENTRY FHL1.
Flow Cytometry: FHL1 Antibody (OTI3G7) - Azide and BSA Free [NBP2-70739]

Flow Cytometry: FHL1 Antibody (OTI3G7) - Azide and BSA Free [NBP2-70739]

Flow Cytometry: FHL1 Antibody (OTI3G7) - Azide and BSA Free [NBP2-70739] - Analysis of Jurkat cells, using anti-FHL1 antibody, (Red), compared to a nonspecific negative control antibody (Blue).
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Applications for FHL1 Antibody (OTI3G7) - Azide and BSA Free

Application
Recommended Usage

Flow Cytometry

1:100

Immunocytochemistry/ Immunofluorescence

1:100

Western Blot

1:500-2000

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Reconstitution

we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process.

Formulation

Lyophilized from PBS (pH 7.3) with 8% Trehalose

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

LYOPH mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: FHL1

LIM proteins, named for 'LIN11, ISL1, and MEC3,' are defined by the possession of a highly conserved double zinc finger motif called the LIM domain.[supplied by OMIM]

Long Name

Four and a Half LIM Domains Protein 1

Alternate Names

HLH1, HPLH1, KYOT, SLIM1, SLIMMER, XMPMA

Gene Symbol

FHL1

Additional FHL1 Products

Product Documents for FHL1 Antibody (OTI3G7) - Azide and BSA Free

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for FHL1 Antibody (OTI3G7) - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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