FHL1 Antibody
Novus Biologicals, part of Bio-Techne | Catalog # NB100-1460
Key Product Details
Species Reactivity
Validated:
Mouse, Rat
Cited:
Mouse
Applications
Validated:
Immunohistochemistry, Immunohistochemistry-Paraffin, Peptide ELISA, Western Blot
Cited:
Immunohistochemistry-Paraffin, Western Blot
Label
Unconjugated
Antibody Source
Polyclonal Goat IgG
Concentration
0.5 mg/ml
Product Specifications
Immunogen
Peptide with sequence CNKRFVFHNEQVY, from the internal region of the protein sequence according to NP_001070830.1; NP_001274729.1.
Reactivity Notes
Use in Mouse reported in scientific literature (PMID:18056057).
Specificity
This antibody is expected to recognize Mouse NP_001070830.1 iso 2 , NP_001274729.1 iso 3 and Rat NP_001029098.1 iso 1, NP_001258128.1 iso 3. Since June 2009 the second N residue in the immunizing peptide is no longer representative as it has been substituted by Q at position 284 of NP_001440.2. Variants NP_001440.2, NP_001153176.1 and NP_001153172.1 represent identical protein.
Clonality
Polyclonal
Host
Goat
Isotype
IgG
Scientific Data Images for FHL1 Antibody
Western Blot: FHL1 Antibody [NB100-1460] -
Western Blot: FHL1 Antibody [NB100-1460] - (0.1ug/ml) staining of Mouse Skeletal Muscle (A), Rat Lung (B) and Rat Skeletal Muscle (C) lysate (35ug protein in RIPA buffer). Detected by chemiluminescence.Applications for FHL1 Antibody
Application
Recommended Usage
Peptide ELISA
Detection limit 1:32000
Western Blot
0.05 - 0.3 ug/mL
Application Notes
WB: Approx 32kDa band observed in Mouse and Rat Skeletal Muscle and in Rat Lung lysates (calculated MW of 31.9kDa according to Mouse NP_001274729.1 and Rat NP_001258128.1). Primary incubation 1 hour at room temperature.
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Immunogen affinity purified
Formulation
Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA
Preservative
0.02% Sodium Azide
Concentration
0.5 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at -20C. Avoid freeze-thaw cycles.
Background: FHL1
Long Name
Four and a Half LIM Domains Protein 1
Alternate Names
HLH1, HPLH1, KYOT, SLIM1, SLIMMER, XMPMA
Gene Symbol
FHL1
UniProt
Additional FHL1 Products
Product Documents for FHL1 Antibody
Product Specific Notices for FHL1 Antibody
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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