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FHL1 Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-88745

Novus Biologicals, part of Bio-Techne
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NBP1-88745
NBP1-88745-25ul

Key Product Details

Validated by

Orthogonal Validation

Species Reactivity

Validated:

Human

Predicted:

Mouse (90%), Rat (91%). Backed by our 100% Guarantee.

Applications

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Product Specifications

Immunogen

This antibody was developed against Recombinant Protein corresponding to amino acids: KFCANTCVECRKPIGADSKEVHYKNRFWHDTCFRCAKCLHPLANETFVAKDNKILCNKCTTREDSPKCKGCFKAIVAGDQNVEYKGTVWHKDCFTCSNCKQV

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for FHL1 Antibody

Immunohistochemistry-Paraffin: FHL1 Antibody [NBP1-88745]

Immunohistochemistry-Paraffin: FHL1 Antibody [NBP1-88745]

Immunohistochemistry-Paraffin: FHL1 Antibody [NBP1-88745] - Analysis human skeletal muscle and pancreas tissues. Corresponding FHL1 RNA-seq data are presented for the same tissues
Western Blot: FHL1 Antibody [NBP1-88745]

Western Blot: FHL1 Antibody [NBP1-88745]

Western Blot: FHL1 Antibody [NBP1-88745] - Analysis in human skeletal muscle tissue.
Immunocytochemistry/ Immunofluorescence: FHL1 Antibody [NBP1-88745]

Immunocytochemistry/ Immunofluorescence: FHL1 Antibody [NBP1-88745]

Immunocytochemistry/Immunofluorescence: FHL1 Antibody [NBP1-88745] - Staining of human cell line U-251 MG shows localization to cytosol. Antibody staining is shown in green.

Applications for FHL1 Antibody

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

0.25-2 ug/ml

Immunohistochemistry

1:200 - 1:500

Immunohistochemistry-Paraffin

1:200 - 1:500

Western Blot

0.04-0.4 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. ICC/IF Fixation Permeabilization: Use PFA/Triton X-100.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: FHL1

LIM proteins, named for 'LIN11, ISL1, and MEC3,' are defined by the possession of a highly conserved double zinc finger motif called the LIM domain.[supplied by OMIM]

Long Name

Four and a Half LIM Domains Protein 1

Alternate Names

HLH1, HPLH1, KYOT, SLIM1, SLIMMER, XMPMA

Entrez Gene IDs

2273 (Human)

Gene Symbol

FHL1

UniProt

Additional FHL1 Products

Product Documents for FHL1 Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for FHL1 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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