FHL2 Antibody (AT21D11) - BSA Free
Novus Biologicals, part of Bio-Techne | Catalog # NBP2-61162
![Western Blot: FHL2 Antibody (AT21D11)BSA Free [NBP2-61162]](https://resources.bio-techne.com/images/products/FHL2-Antibody-AT21D11-Western-Blot-NBP2-61162-img0001.jpg "Western Blot: FHL2 Antibody (AT21D11)BSA Free [NBP2-61162]")
Conjugate
Catalog #
Key Product Details
Species Reactivity
Validated:
Human
Applications
ELISA, Immunocytochemistry/ Immunofluorescence, Western Blot
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG1 kappa Clone # AT21D11
Format
BSA Free
Concentration
1 mg/ml
Product Summary for FHL2 Antibody (AT21D11) - BSA Free
Immunogen
Recombinant human FHL2 (1-279aa) purified from E. coli.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG1 kappa
Scientific Data Images for FHL2 Antibody (AT21D11) - BSA Free
Western Blot: FHL2 Antibody (AT21D11)BSA Free [NBP2-61162]
Western Blot: FHL2 Antibody (AT21D11) [NBP2-61162] - The cell lysates(40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human FHL2 antibody (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.Lane 1.: HeLa cell lysateLane 2.: A549 cell lysate Lane 3.: MCF7 cell lysate Lane 4.: SK-OV-3 cell lysateLane 5.: HT1080 cell lysate![Immunocytochemistry/ Immunofluorescence: FHL2 Antibody (AT21D11) - BSA Free [NBP2-61162]](https://resources.bio-techne.com/images/products/FHL2-Antibody-AT21D11-Immunocytochemistry-Immunofluorescence-NBP2-61162-img0002.jpg "Immunocytochemistry/ Immunofluorescence: FHL2 Antibody (AT21D11) - BSA Free [NBP2-61162]")
Immunocytochemistry/ Immunofluorescence: FHL2 Antibody (AT21D11) - BSA Free [NBP2-61162]
Immunocytochemistry/Immunofluorescence: FHL2 Antibody (AT21D11) [NBP2-61162] - FHL2 in HeLa cells. The cell was stained with ATGA0486 (1:100). The secondary antibody (green) was used Alexa Fluor 488. DAPI was stained the cell nucleus (blue).Applications for FHL2 Antibody (AT21D11) - BSA Free
Application
Recommended Usage
ELISA
1:100-1:2000
Immunocytochemistry/ Immunofluorescence
1:10-1:500
Western Blot
1:100-1:2000
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Protein A purified
Formulation
PBS (pH 7.4), 10% Glycerol
Format
BSA Free
Preservative
0.02% Sodium Azide
Concentration
1 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Background: FHL2
Long Name
Four and a Half LIM Domains Protein 2
Alternate Names
AAG11, DRAL, SLIM3
Gene Symbol
FHL2
Additional FHL2 Products
Product Documents for FHL2 Antibody (AT21D11) - BSA Free
Product Specific Notices for FHL2 Antibody (AT21D11) - BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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