Fibrin beta-chain Antibody (8E5) - Chimeric - Azide and BSA Free
Novus Biologicals, part of Bio-Techne | Catalog # NBP2-81111
Recombinant Monoclonal Antibody
![Flow Cytometry: Fibrin beta-chain Antibody (8E5) - Chimeric - Azide and BSA Free [NBP2-81111]](https://resources.bio-techne.com/images/products/Fibrinopeptide-B-Antibody-8E5-Chimeric-Flow-Cytometry-NBP2-81111-img0001.jpg "Flow Cytometry: Fibrin beta-chain Antibody (8E5) - Chimeric - Azide and BSA Free [NBP2-81111]")
Conjugate
Catalog #
Key Product Details
Species Reactivity
Human
Applications
ELISA, Flow Cytometry, In vivo assay
Label
Unconjugated
Antibody Source
Recombinant Monoclonal Rabbit IgG Kappa Clone # 8E5
Format
Azide and BSA Free
Concentration
1 mg/ml
Product Specifications
Immunogen
8E5 was prepared fusing Sp2/o myeloma cells with spleen cells derived from female BALB/c mice immunized with a synthetic beta-fibrin peptide seqeunce GHRPLDKC conjugated to MB-KLH (maleimidobenzoylated keyhole limpet hemocyanin).
Specificity
8E5 binds to the amino-terminal region of the beta-chain of human fibrin at an epitope consiting of GHRPLDKC. Binding is possible in both the monomeric form and the polymeric form of the protein. This peptide sequence is found only in fibrin, and not in its precursor fibrinogen and so there is no cross-reactivity with fibrinogen. Fibrin is a non-globular protein which plays a role in the clotting of blood and circulates as the precursor fibrinogen. Polymerization occurs through the action of the protease thrombin, which results in the active fibrin. Fibrin polymerizes as an insoluble matrix and forms a clot along with platelets over a wound site.
Clonality
Monoclonal
Host
Rabbit
Isotype
IgG Kappa
Scientific Data Images for Fibrin beta-chain Antibody (8E5) - Chimeric - Azide and BSA Free
Flow Cytometry: Fibrin beta-chain Antibody (8E5) - Chimeric - Azide and BSA Free [NBP2-81111]
Flow Cytometry: Fibrin beta-chain Antibody (8E5) - Chimeric [NBP2-81111] - Flow-cytometry using the Fibrin beta-chain Antibody [NBP2-81111]. HepG2 cells were fixed using 2% PFA, permeabilised using 0.5% Triton and stained with anti-Fluorescein IgG antibody (isotype control, black line) or the rabbit IgG-chimeric version of 8E5 (NBP2-81111, blue line) at a dilution of 1:100 for 1h at RT. After washing, bound antibody was detected using a goat anti-rabbit IgG AlexaFluor 488 antibody at a dilution of 1:1000 and cells analyzed using a FACSCanto flow-cytometer.Applications for Fibrin beta-chain Antibody (8E5) - Chimeric - Azide and BSA Free
Application
Recommended Usage
ELISA
Optimal dilutions of this antibody should be experimentally determined.
Flow Cytometry
Optimal dilutions of this antibody should be experimentally determined.
In vivo assay
Optimal dilutions of this antibody should be experimentally determined.
Application Notes
This chimeric rabbit antibody was made using the variable domain sequences of the original Mouse IgG1 format, for improved compatibility with existing reagents, assays and techniques. The recombinant scFv version of 8E5 retains the same antigen-binding activity of the parent antibody. 8E5 can bind to fibrin in the monomeric and polymeric form, both in vivo and ex vivo. This antibody can also be used for the immunodetection of fibrin in vivo. Standard ELISA and competitive ELISA using the epitope peptide has been conducted with both the parental antibody and the scFv.
Formulation, Preparation, and Storage
Purification
Protein A purified
Formulation
PBS
Format
Azide and BSA Free
Preservative
0.02% Proclin 300
Concentration
1 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C for up to 3 months. For longer storage, aliquot and store at -20C.
Background: Fibrin beta-chain
Alternate Names
Beta-Fibrinogen, epididymis secretory sperm binding protein Li 78p, fibrinogen beta chain, fibrinogen, B beta polypeptide, HEL-S-78p
Gene Symbol
FGB
Additional Fibrin beta-chain Products
Product Documents for Fibrin beta-chain Antibody (8E5) - Chimeric - Azide and BSA Free
Product Specific Notices for Fibrin beta-chain Antibody (8E5) - Chimeric - Azide and BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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