Fibrinogen Antibody
Novus Biologicals, part of Bio-Techne | Catalog # NBP2-50087
Key Product Details
Species Reactivity
Validated:
Human
Cited:
Human, Mouse
Applications
Validated:
ELISA, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot
Cited:
Immunofluorescence, Immunohistochemistry, Western Blot
Label
Unconjugated
Antibody Source
Polyclonal Goat IgG
Concentration
LYOPH mg/ml
Product Specifications
Immunogen
Fibrinogen [Human Plasma] (Uniprot: P02671)
Reactivity Notes
Cross reactivity against Fibrinogen from other sources is unknown
Clonality
Polyclonal
Host
Goat
Isotype
IgG
Description
This antibody is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Goat Serum as well as purified and partially purified Fibrinogen [Human Plasma]
Store vial at 4C prior to restoration. For extended storage aliquot contents and freeze at -20C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4C as an undiluted liquid. Dilute only prior to immediate use.
Store vial at 4C prior to restoration. For extended storage aliquot contents and freeze at -20C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4C as an undiluted liquid. Dilute only prior to immediate use.
Scientific Data Images for Fibrinogen Antibody
Western Blot: Fibrinogen Antibody [NBP2-50087]
Western Blot: Fibrinogen Antibody [NBP2-50087] - Lane 1: Fibrinogen under reducing conditions. Lane 2: none. Load: 1 ug per lane. Primary antibody: Fibrinogen antibody at 1:3000 for overnight at 4C. Secondary antibody: Dylight 488 conjugated Donkey anti goat secondary antibody at 1:10,000 for 45 min at RT. Block: TBS/blocking buffer @ 1 hr RT.Immunohistochemistry-Paraffin: Fibrinogen Antibody [NBP2-50087]
Immunohistochemistry-Paraffin: Fibrinogen Antibody [NBP2-50087] - Tissue: human liver tissue. Fixation: formalin fixed paraffin embedded. Antigen retrieval: not required. Primary antibody: Fibrinogen antibody at 1:500 for 1 h at RT. Secondary antibody: Peroxidase goat secondary antibody at 1:10,000 for 45 min at RT. Localization: Fibrinogen is localized in plasma. Staining: Fibrinogen as precipitated red signal with hematoxylin purple nuclear counterstain.Fibrinogen Antibody
Immunohistochemistry of Goat Anti-Fibrinogen antibody. Tissue: human liver tissue. Fixation: formalin fixed paraffin embedded. Antigen retrieval: not required. Primary antibody: Fibrinogen antibody at 1:500 for 1 h at RT. Secondary antibody: Peroxidase goat secondary antibody at 1:10,000 for 45 min at RT. Localization: Fibrinogen is localized in plasma. Staining: Fibrinogen as precipitated red signal with hematoxylin purple nuclear counterstain.Applications for Fibrinogen Antibody
Application
Recommended Usage
ELISA
1:20000-1:100000
Immunohistochemistry
1:500
Immunohistochemistry-Paraffin
1:500
Western Blot
1:2000-1:10000
Application Notes
This product has been tested in western blot and immunohistochemistry. This antibody is suitable when assayed against 1.0 ug of Fibrinogen [Human Plasma] in a standard ELISA using Peroxidase conjugated Affinity Purified anti-Goat IgG [H&L] (Rabbit) and (ABTS (2,2'-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) as a substrate for 30 minutes at room temperature. A working dilution of 1:10,000 to 1:50,000 of the reconstitution concentration is suggested for this product. Specific conditions for reactivity should be optimized by the end user.
Formulation, Preparation, and Storage
Purification
Multi-step
Reconstitution
Reconstitute with 100 ul deionized water (or equivalent)
Formulation
Lyophilized from 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Preservative
0.01% Sodium Azide
Concentration
LYOPH mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store lyophilized antibody at 4C. Aliquot reconstituted liquid and store at -20C. Avoid freeze-thaw cycles.
Background: Fibrinogen
Additional Fibrinogen Products
Product Documents for Fibrinogen Antibody
Product Specific Notices for Fibrinogen Antibody
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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