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Fibroblast Activation Protein alpha/FAP Antibody (FAP/4853) - Azide and BSA Free

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-14220

Novus Biologicals, part of Bio-Techne

Key Product Details

Species Reactivity

Validated:

Human

Applications

Immunohistochemistry-Paraffin, Protein Array

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2c Kappa Clone # FAP/4853

Format

Azide and BSA Free

Concentration

1 mg/ml

Product Summary

Immunogen

Recombinant fragment (around aa 1-200) of human Fibroblast Activation Protein alpha/FAP protein (exact sequence is proprietary) (Uniprot: Q12884)

Localization

Cell surface, Cell membrane

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2c Kappa

Theoretical MW

88 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Description

1.0 mg/ml of antibody purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS WITHOUT BSA & azide. Also available at 200 ug/ml WITH BSA & azide (NBP3-13884).

Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80 C.

Scientific Data Images

Protein Array: Fibroblast Activation Protein alpha/FAP Antibody (FAP/4853) - Azide and BSA Free [NBP3-14220]

Protein Array: Fibroblast Activation Protein alpha/FAP Antibody (FAP/4853) - Azide and BSA Free [NBP3-14220]

Protein Array: Fibroblast Activation Protein alpha/FAP Antibody (FAP/4853) - Azide and BSA Free [NBP3-14220] - Analysis of Protein Array containing more than 19,000 full-length human proteins using Fibroblast Activation Protein alpha/FAP Antibody (FAP/4853).

Applications

Application
Recommended Usage

Immunohistochemistry-Paraffin

1-2 ug/ml
Application Notes
Immunohistochemistry (Formalin-fixed): 1-2ug/ml for 30 minutes at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95C followed by cooling at RT for 20 minutes
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Protein A or G purified

Formulation

10mM PBS

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20 to -80C. Avoid freeze-thaw cycles.

Background: Fibroblast Activation Protein alpha/FAP

FAP (fibroblast activation protein-alpha; also known as seprase) is a type II transmembrane serine protease that degrades gelatin, Type I collagen, and N-terminal prolines. FAP is induced on reactive stromal fibroblasts in epithelial cancers, sarcomas, and granulation tissue, and may play roles in tumor invasion, tissue remodeling, and wound repair.

Alternate Names

FAP, Seprase

Gene Symbol

FAP

Additional Fibroblast Activation Protein alpha/FAP Products

Product Documents

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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