Key Product Details

Species Reactivity

Human, Mouse

Applications

ELISA, Flow Cytometry, Western Blot

Label

CoraFluor 1

Antibody Source

Polyclonal Rabbit IgG

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

View all Fibroblast Activation Protein alpha/FAP Antibodies »

Product Specifications

Immunogen

This affinity purified Fibroblast Activation Protein alpha/FAP Antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to an internal region of mouse Fibroblast Activation Protein alpha/Fibroblast Activation Protein alpha/FAP protein. (Uniprot: P97321)

Reactivity Notes

A BLAST analysis was used to suggest partial cross-reactivity with Fibroblast Activation Protein alpha/FAP from human and rat sources based on ~88% homology with the immunizing sequence. Reactivity with Fibroblast Activation Protein alpha/FAP from other sources has not been determined. Human reactivity reported from a verified customer review.

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Description

CoraFluor(TM) 1 is a high performance terbium-based TR-FRET (Time-Resolved Fluorescence Resonance Energy Transfer) or TRF (Time-Resolved Fluorescence) donor for high throughput assay development. CoraFluor(IM) 1 absorbs UV light at approximately 340 nm, and emits at approximately 490 nm, 545 nm, 585 nm and 620 nm. It is compatible with common acceptor dyes that absorb at the emission wavelengths of CoraFluor(TM) 1. CoraFluor(TM) 1 can be used for the development of robust and scalable TR-FRET binding assays such as target engagement, ternary complex, protein-protein interaction and protein quantification assays.

Scientific Data Images for Fibroblast Activation Protein alpha/FAP Antibody [CoraFluor™ 1]

Fibroblast Activation Protein alpha/FAP Antibody [CoraFluor™ 1]

Flow Cytometry: Fibroblast Activation Protein alpha/FAP Antibody [CoraFluor™ 1] [NB110-85534CL1]

Flow Cytometry: Fibroblast Activation Protein alpha/FAP Antibody [CoraFluor™ 1] [NB110-85534CL1] - Specific co-culture system created to study human macrophage cancer cells' conduction of MMT process, and Fibroblast Activation Protein alpha/FAP Antibody [Alexa Fluor® 488] (Catalog # NB110-85534AF488) was used as a myo-CAFs marker. Image from a verified customer review.

Applications for Fibroblast Activation Protein alpha/FAP Antibody [CoraFluor™ 1]

Application
Recommended Usage

Flow Cytometry

Validated for Flow Cytometry from a verified customer review.
Application Notes
Optimal dilution of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS

Preservative

No Preservative

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C in the dark. Do not freeze.

Background: Fibroblast Activation Protein alpha/FAP

FAP (fibroblast activation protein-alpha; also known as seprase) is a type II transmembrane serine protease that degrades gelatin, Type I collagen, and N-terminal prolines. FAP is induced on reactive stromal fibroblasts in epithelial cancers, sarcomas, and granulation tissue, and may play roles in tumor invasion, tissue remodeling, and wound repair.

Alternate Names

FAP, Seprase

Gene Symbol

FAP

Additional Fibroblast Activation Protein alpha/FAP Products

Product Documents for Fibroblast Activation Protein alpha/FAP Antibody [CoraFluor™ 1]

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Download SDS

Product Specific Notices for Fibroblast Activation Protein alpha/FAP Antibody [CoraFluor™ 1]

CoraFluor (TM) is a trademark of Bio-Techne Corp. Sold for research purposes only under agreement from Massachusetts General Hospital. US patent 2022/0025254

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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