Fibroblasts/Epithelial cells Antibody (D7-FIB)
Novus Biologicals, part of Bio-Techne | Catalog # NB600-777
![Flow Cytometry: Fibroblasts/Epithelial cells Antibody (D7-FIB) - BSA Free [NB600-777]](https://resources.bio-techne.com/images/products/Fibroblasts-Epithelial-cells-Antibody-D7-FIB-Flow-Cytometry-NB600-777-img0001.jpg "Flow Cytometry: Fibroblasts/Epithelial cells Antibody (D7-FIB) - BSA Free [NB600-777]")
Conjugate
Catalog #
Key Product Details
Species Reactivity
Human
Applications
Validated:
Flow Cytometry, Immunohistochemistry, Immunohistochemistry-Frozen, Immunohistochemistry-Paraffin (Negative), Immunoprecipitation
Cited:
Immunocytochemistry/ Immunofluorescence
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG2A Clone # D7-FIB
Concentration
1.0 mg/ml
Product Specifications
Immunogen
Human foreskin fibroblasts
Localization
Cell Membrane
Specificity
NB600-777 recognizes a 112kD molecule expressed on the cell surface of human fibroblasts and epithelial cells. In peripheral blood the antibody stains myeloid cells and a very small number of lymphocytes. Studies upon the antigen have shown it to be sensitive to SDS, but resistant to trypsin, tunicamycin and collagenase. In immunohistological studies the antibody has also been found to bind to epithelium, myoepthelium, smooth muscle and some leucocytes. D7-FIB has been shown to be useful as a cell membrane marker to characterize chondrocyte differentiation giving a positive reaction with dedifferentiated human chondrocytes, and negative with differentiated chondrocytes (3). This product is routinely tested in flow cytometry on the KG1 cell line.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG2A
Description
Mouse anti Human Fibroblasts/Epithelial Cells antibody, clone D7-FIB recognizes a ~112kDa molecule expressed on the cell surface of human fibroblasts. The antigen is not expressed by peripheral blood cells, and is found at low levels on a minority of melanoma cell lines. Studies upon the antigen have shown it to be sensitive to SDS, but resistant to trypsin, tunicamycin and collagenase.
D7-FIB has also been found to bind to epithelium, myoepthelium, smooth muscle and some leucocytes. D7-FIB is useful as a cell membrane marker to characterize chondrocyte differentiation giving a positive reaction with dedifferentiated human chondrocytes, and negative with differentiated chondrocytes (van Osch et al. 2001).
Scientific Data Images for Fibroblasts/Epithelial cells Antibody (D7-FIB)
Flow Cytometry: Fibroblasts/Epithelial cells Antibody (D7-FIB) - BSA Free [NB600-777]
Flow Cytometry: Fibroblasts/Epithelial cells Antibody (D7-FIB) [NB600-777] - Fibroblast Marker Staining of KG1 cells.Applications for Fibroblasts/Epithelial cells Antibody (D7-FIB)
Application
Recommended Usage
Flow Cytometry
1:50-1:200
Immunohistochemistry-Frozen
1:100
Immunoprecipitation
1:10-1:500
Application Notes
Flow Cytometry: Use 10 ul of the suggested working dilution to label 10^6 cells in 100 ul. The epitope recognised by this antibody is reported to be sensitive to formaldehyde fixation and tissue processing. The use of acetone fixation for frozen sections is recommended. This antibody does not work in Immunohistochemistry-Paraffin. Use in Immunocytochemistry/immunofluorescence reported in scientific literature (PMID 27416040).
Formulation, Preparation, and Storage
Purification
Protein A purified
Formulation
PBS
Preservative
0.09% Sodium Azide
Concentration
1.0 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Background: Fibroblasts/Epithelial cells
Alternate Names
fibro-blasts
Additional Fibroblasts/Epithelial cells Products
Product Documents for Fibroblasts/Epithelial cells Antibody (D7-FIB)
Product Specific Notices for Fibroblasts/Epithelial cells Antibody (D7-FIB)
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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