Fibronectin Antibody (568) - Azide and BSA Free
Novus Biologicals, part of Bio-Techne | Catalog # NBP2-48018
![Immunohistochemistry-Paraffin: Fibronectin Antibody (568) - Azide and BSA Free [NBP2-48018]](https://resources.bio-techne.com/images/products/Fibronectin-Antibody-568-Azide-and-BSA-Free-Immunohistochemistry-Paraffin-NBP2-48018-img0001.jpg "Immunohistochemistry-Paraffin: Fibronectin Antibody (568) - Azide and BSA Free [NBP2-48018]")
Conjugate
Catalog #
Forumulation
Catalog #
Key Product Details
Species Reactivity
Human
Applications
CyTOF-ready, Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG1 kappa Clone # 568
Format
Azide and BSA Free
Concentration
1.0 mg/ml
Product Specifications
Immunogen
High molecular weight proteins secreted by cultivated human fibroblasts (Uniprot: P02751)
Reactivity Notes
Use in Human reported in scientific literature (PMID:34474855)
Localization
Connective tissue matrix
Specificity
Epitope of this monoclonal antibody is located in the 8th type III repeat in the cell-binding region of fibronectin. Fibronectins are disulfide-linked, dimeric glycoproteins of ~440kDa. They possess at least four binding sites for collagen, glycosaminoglycans, transglutaminase, and a cell surface receptor. Fibronectins are involved in cell adhesion, tissue organization, and wound healing. Fibronectins are present in basement membranes, interstitial connective tissue matrix, and blood. Cellular fibronectin is widely distributed in the stroma of many malignant tumors. This monoclonal antibody is excellent for staining of formalin-fixed, paraffin-embedded tissues.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG1 kappa
Description
1.0 mg/ml of antibody purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS WITHOUT BSA & azide. Also available at 200 ug/ml WITH BSA & azide (NBP2-45018).
Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80C.
Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80C.
Scientific Data Images for Fibronectin Antibody (568) - Azide and BSA Free
Immunohistochemistry-Paraffin: Fibronectin Antibody (568) - Azide and BSA Free [NBP2-48018]
Immunohistochemistry-Paraffin: Fibronectin Antibody (568) - Azide and BSA Free [NBP2-48018] - Human Renal Cell Carcinoma stained with Fibronectin Monoclonal Antibody (568).Applications for Fibronectin Antibody (568) - Azide and BSA Free
Application
Recommended Usage
Flow Cytometry
0.5 - 1 ug/million cells in 0.1 ml
Immunocytochemistry/ Immunofluorescence
1-2 ug/ml
Immunofluorescence
0.5 - 1.0 ug/ml
Immunohistochemistry-Paraffin
1 - 2 ug/ml
Application Notes
Immunohistochemistry (Formalin-fixed): 1-2ug/ml for 30 minutes at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95C followed by cooling at RT for 20 minutes.
Optimal dilution for a specific application should be determined.
Optimal dilution for a specific application should be determined.
Formulation, Preparation, and Storage
Purification
Protein A or G purified
Formulation
10 mM PBS
Format
Azide and BSA Free
Preservative
No Preservative
Concentration
1.0 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at -20 to -80C. Avoid freeze-thaw cycles.
Background: Fibronectin
Alternate Names
CIG, ED-B, FINC, FN1, FNZ, GFND2, LETS, SMDCF
Gene Symbol
FN1
Additional Fibronectin Products
Product Documents for Fibronectin Antibody (568) - Azide and BSA Free
Product Specific Notices for Fibronectin Antibody (568) - Azide and BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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