Fibronectin Antibody (A17) - BSA Free
Novus Biologicals, part of Bio-Techne | Catalog # NBP2-23524
Conjugate
Catalog #
Key Product Details
Species Reactivity
Human, Bovine, Chicken
Applications
ELISA, Immunohistochemistry, Immunohistochemistry-Frozen, Immunohistochemistry-Paraffin, Immunoprecipitation, Western Blot
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG1 Clone # A17
Format
BSA Free
Concentration
1 mg/ml
Product Specifications
Immunogen
Lysed bovine corneal endothelial cells and extracellular matrix
Epitope
Epitope is located in the 120kD cell binding fragment.
Specificity
Highly specific for fibronectin. There is no evidence for cross-reactivity with other connective tissue proteins (vitronectin, elastin, collagen, laminin). The antibody inhibits integrin-mediated cell adhesion to the cell binding domain of fibronectin.
Marker
Mesenchymal Cells Marker
Clonality
Monoclonal
Host
Mouse
Isotype
IgG1
Applications for Fibronectin Antibody (A17) - BSA Free
Application
Recommended Usage
ELISA
1:100-1:2000
Immunohistochemistry
1:10-1:500
Immunoprecipitation
1:10-1:500
Western Blot
1:100-1:2000
Application Notes
ELISA: NBP2-23524 can be used in ELISA. (1, 2, 3, 4, 5)
WB: In Western blotting a dilution guideline of 1/100 has proved successful. (1)
IHC: NBP2-23524 can be used in immunostaining of frozen PLP-fixed sections of bovine and human tissues.
IP: NBP2-23524 can be used in immunopercipitation. It can be used to probe fibronectin conformation.
Formulation, Preparation, and Storage
Purification
Protein A or G purified
Formulation
10mM Phosphate (pH 7.4) and 0.5M NaCl
Format
BSA Free
Preservative
0.9% Sodium Azide
Concentration
1 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C in the dark.
Background: Fibronectin
Alternate Names
CIG, ED-B, FINC, FN1, FNZ, GFND2, LETS, SMDCF
Gene Symbol
FN1
Additional Fibronectin Products
Product Documents for Fibronectin Antibody (A17) - BSA Free
Product Specific Notices for Fibronectin Antibody (A17) - BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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