Fibronectin Antibody (A32) - BSA Free

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-23525

Novus Biologicals, part of Bio-Techne
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NBP2-23525-0.1ml
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Key Product Details

Species Reactivity

Human, Bovine

Applications

ELISA, Immunohistochemistry, Immunohistochemistry-Frozen, Immunohistochemistry-Paraffin, Immunoprecipitation, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # A32

Format

BSA Free

Concentration

1 mg/ml

View all Fibronectin Antibodies »

Product Specifications

Immunogen

Bovine corneal endothelial cells

Epitope

Epitope is located in the 40kD Hep II heparin-binding domain, but differs from that of Clone A32

Specificity

NBP2-23525 is highly specific for fibronectin. There is no evidence for cross-reactivity with other connective tissue proteins (vitronectin, elastin, collagen, laminin). The antibody inhibits cell adhesion to fibronectin mediated by the heparin-binding domain.

Marker

Mesenchymal Cells Marker

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Applications for Fibronectin Antibody (A32) - BSA Free

Application
Recommended Usage

ELISA

1:100-1:2000

Immunohistochemistry

1:10-1:500

Immunoprecipitation

1:10-1:500

Western Blot

1:100-1:2000
Application Notes
ELISA: NBP2-23525 can be used in ELISA. It can be used to probe fibronectin conformation and to quantitate plasma fibronectin in a sandwich ELISA with antibody NBP1-05056. (1, 2, 3, 4) WB: In Western blotting dilution guideline of 1/100 has proved successful. (1) IHC: NBP2-23525 can be used in immunostaining of frozen PLP-fixed sections of bovine and human tissues. IP: NBP2-23525 can be used in immunoprecipitation.

Formulation, Preparation, and Storage

Purification

Protein A or G purified

Formulation

10mM Phosphate (pH 7.4) and 0.5M NaCl

Format

BSA Free

Preservative

0.09% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C in the dark.

Background: Fibronectin

Fibronectin is an adhesive glycoprotein with a molecular mass of 440 kDa. It is believed to be important for the formation of a provisional matrix that promotes cell adhesion and migration during wound healing. Its age-dependent increase in plasma and tissues may be accompanied in pathological states, especially in tumor growth, by its proteolytic breakdown by a number of neutral proteases. It has also shown that several of its proteolytic breakdown products exhibit unexpected and mostly harmful biological activities

Alternate Names

CIG, ED-B, FINC, FN1, FNZ, GFND2, LETS, SMDCF

Gene Symbol

FN1

Additional Fibronectin Products

Product Documents for Fibronectin Antibody (A32) - BSA Free

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for Fibronectin Antibody (A32) - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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