Fibronectin Antibody (CL3730)

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-61633

Novus Biologicals, part of Bio-Techne
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NBP2-61633
NBP2-61633-25ul
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Key Product Details

Species Reactivity

Validated:

Human

Predicted:

Mouse (95%), Rat (95%). Backed by our 100% Guarantee.

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # CL3730

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

View all Fibronectin Antibodies »

Product Specifications

Immunogen

This antibody was developed against a recombinant protein corresponding to amino acids: HEEICTTNEGVMYRIGDQWDKQHDMGHMMRCTCVGNGRGEWTCIAYSQLRDQCIVDDITYNVNDTFHKRHEEGHMLNCTCFGQGRGRWKCDPVDQCQDSETGTFYQIGDSWEKYVHGVRYQCYCYGRGIG

Epitope

GRWKCDPVDQ

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any questions

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Scientific Data Images for Fibronectin Antibody (CL3730)

Western Blot: Fibronectin Antibody (CL3730) [NBP2-61633]

Western Blot: Fibronectin Antibody (CL3730) [NBP2-61633]

Western Blot: Fibronectin Antibody (CL3730) [NBP2-61633] - Lane 1: Marker [kDa] 250, 130, 100, 70, 55, 35, 25, 15, 10 Lane 2: Human cell line U-87 MG
Immunohistochemistry-Paraffin: Fibronectin Antibody (CL3730) [NBP2-61633]

Immunohistochemistry-Paraffin: Fibronectin Antibody (CL3730) [NBP2-61633]

Immunohistochemistry-Paraffin: Fibronectin Antibody (CL3730) [NBP2-61633] - Staining of human pancreas shows absence of immunoreactivity in glandular cells (negative control).
Immunohistochemistry-Paraffin: Fibronectin Antibody (CL3730) [NBP2-61633]

Immunohistochemistry-Paraffin: Fibronectin Antibody (CL3730) [NBP2-61633]

Immunohistochemistry-Paraffin: Fibronectin Antibody (CL3730) [NBP2-61633] - Staining of human endometrium shows strong immunoreactivity in endometrial stroma and basement membrane of glandular cells.
View 6 more images

Applications for Fibronectin Antibody (CL3730)

Application
Recommended Usage

Immunohistochemistry

1:1000 - 1:2500

Immunohistochemistry-Paraffin

1:1000 - 1:2500

Western Blot

1 ug/ml
Application Notes
IHC-Paraffin HIER pH6 retrieval is recommended.
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Fibronectin

Fibronectin (also known as FN1, or Fibronectin 1) is an extracellular matrix glycoprotein that binds to integrins and has a large role in cell adhesion, migration, differentiation, and growth. It plays a key role in wound healing by helping form blood clots and participates in embryonic development by guiding cell attachment and migration. Fibronectin consists of two monomers linked by disulfide bonds and binds extracellular components such as collagen fibrin and syndecans. Fibronectin has been implicated in carcinoma and tumor development while also limiting tumor cells response to treatment. Novus offers Fibronectin antibodies, ELISA Kits, lysates, and proteins

Alternate Names

CIG, ED-B, FINC, FN1, FNZ, GFND2, LETS, SMDCF

Gene Symbol

FN1

Additional Fibronectin Products

Product Documents for Fibronectin Antibody (CL3730)

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for Fibronectin Antibody (CL3730)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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