Fibulin-3/EFEMP1 Antibody (OTI1D9) - Azide and BSA Free

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-70700

Novus Biologicals, part of Bio-Techne
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Key Product Details

Species Reactivity

Human, Mouse, Rat

Applications

CyTOF-ready, Flow Cytometry, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2A Clone # OTI1D9

Format

Azide and BSA Free

Concentration

LYOPH mg/ml

View all Fibulin 3 Antibodies »

Product Specifications

Immunogen

Full length human recombinant protein of human EFEMP1(NP_004096) produced in HEK293T cell.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2A

Theoretical MW

52.7 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for Fibulin-3/EFEMP1 Antibody (OTI1D9) - Azide and BSA Free

Western Blot: Fibulin-3/EFEMP1 Antibody (OTI1D9)Azide and BSA Free [NBP2-70700]

Western Blot: Fibulin-3/EFEMP1 Antibody (OTI1D9)Azide and BSA Free [NBP2-70700]

Western Blot: Fibulin-3/EFEMP1 Antibody (OTI1D9) - Azide and BSA Free [NBP2-70700] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY EFEMP1 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-EFEMP1.
Flow Cytometry: Fibulin-3/EFEMP1 Antibody (OTI1D9) - Azide and BSA Free [NBP2-70700]

Flow Cytometry: Fibulin-3/EFEMP1 Antibody (OTI1D9) - Azide and BSA Free [NBP2-70700]

Flow Cytometry: Fibulin-3/EFEMP1 Antibody (OTI1D9) - Azide and BSA Free [NBP2-70700] - Analysis of Jurkat cells, using anti-EFEMP1 antibody, (Red), compared to a nonspecific negative control antibody (Blue).

Applications for Fibulin-3/EFEMP1 Antibody (OTI1D9) - Azide and BSA Free

Application
Recommended Usage

Flow Cytometry

1:100

Western Blot

1:200-500

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Reconstitution

we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process.

Formulation

Lyophilized from PBS (pH 7.3) with 8% Trehalose

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

LYOPH mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: Fibulin 3

EFEMP1 spans approximately 18 kb of genomic DNA and consists of 12 exons. Two transcripts with distinct 5' UTR have been described; the resulting proteins have distinct N-terminal amino acid sequences. Translation initiation from internal methionine residues was observed with in vitro translation. A signal peptide sequence is predicted for translation initiation sites 1, 2, and 4. The protein isoforms contain 5 or 6 calcium-binding EGF2 domains and 5 or 6 EGF2 domains. Mutations in this gene cause the retinal disease Malattia Leventinese.

Alternate Names

DHRD, EFEMP1, FBLN3, MLVT

Gene Symbol

EFEMP1

Additional Fibulin 3 Products

Product Documents for Fibulin-3/EFEMP1 Antibody (OTI1D9) - Azide and BSA Free

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for Fibulin-3/EFEMP1 Antibody (OTI1D9) - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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