Fibulin-3/EFEMP1 Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NB300-691

Novus Biologicals, part of Bio-Techne
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NB300-691
NB300-691-0.025mg
NB300-691-0.05mg
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Key Product Details

Species Reactivity

Human, Mouse, Rat, Monkey

Applications

ELISA, Immunohistochemistry, Immunohistochemistry-Paraffin, Immunoprecipitation, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

View all Fibulin 3 Antibodies »

Product Specifications

Immunogen

Synthetic cyclic peptide corresponding to N terminal amino acids 25-42 of Human EFEMP1, peptide was covalently modified. Sequence: TYTQCTDGYEWDPVRQQC

Localization

Secreted

Specificity

The Anti EFEMP1 selective was generated against a conserved sequence near the N terminal region that is unique to EFEMP1 protein only. This detects a strong 62 kDa EFEMP1 protein in human.

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for Fibulin-3/EFEMP1 Antibody

Western Blot: Fibulin-3/EFEMP1 Antibody [NB300-691]

Western Blot: Fibulin-3/EFEMP1 Antibody [NB300-691]

Western Blot: EFEMP1 Antibody [NB300-691] - 62 kDa EFEMP1from human RPE cells. Antibody diluted 1:500
Immunohistochemistry-Paraffin: Fibulin-3/EFEMP1 Antibody [NB300-691]

Immunohistochemistry-Paraffin: Fibulin-3/EFEMP1 Antibody [NB300-691]

Immunohistochemistry-Paraffin: Fibulin-3/EFEMP1 Antibody [NB300-691] - 1:50 dilution, DAB (brown) staining and Hematoxyin QS (blue) counterstain. 40X magnification, FFPE section

Applications for Fibulin-3/EFEMP1 Antibody

Application
Recommended Usage

ELISA

1:10000

Immunohistochemistry-Paraffin

1:100

Immunoprecipitation

1:200

Western Blot

1:500
Application Notes
Detects a band of approximately 62 kDa (predicted molecular weight: 60 kDa). Not tested in other applications.Optimal dilutions/concentrations should be determined by the end user.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

Tris/Glycine buffer, pH 7.4-7.8, HEPES,BSA 0.5%, glycerol 30%.

Preservative

0.02% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Fibulin 3

This gene spans approximately 18 kb of genomic DNA and consists of 12 exons. Two transcripts with distinct 5' UTR have been described; the resulting proteins have distinct N-terminal amino acid sequences. Translation initiation from internal methionine residues was observed with in vitro translation. A signal peptide sequence is predicted for translation initiation sites 1, 2, and 4. The protein isoforms contain 5 or 6 calcium-binding EGF2 domains and 5 or 6 EGF2 domains. Mutations in this gene cause the retinal disease Malattia Leventinese.

Alternate Names

DHRD, EFEMP1, FBLN3, MLVT

Entrez Gene IDs

2202 (Human); 305604 (Rat)

Gene Symbol

EFEMP1

Additional Fibulin 3 Products

Product Documents for Fibulin-3/EFEMP1 Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for Fibulin-3/EFEMP1 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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