Fibulin 5/DANCE Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NB100-53783

Novus Biologicals, part of Bio-Techne
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NB100-53783
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Key Product Details

Species Reactivity

Validated:

Human

Predicted:

Canine (100%), Mouse (100%), Rat (100%). Backed by our 100% Guarantee.

Applications

Peptide ELISA, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Goat IgG

Concentration

0.5 mg/ml

View all Fibulin 5/DANCE Antibodies »

Product Specifications

Immunogen

Peptide with sequence C-RPIKGPREIQLDLE corresponding to internal region (near C-Terminus) according to NP_006320.2.

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Scientific Data Images for Fibulin 5/DANCE Antibody

Western Blot: Fibulin 5/DANCE Antibody [NB100-53783]

Western Blot: Fibulin 5/DANCE Antibody [NB100-53783]

Western Blot: Fibulin 5/DANCE Antibody [NB100-53783] - (0.1ug/ml) staining of Human Ovary lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

Applications for Fibulin 5/DANCE Antibody

Application
Recommended Usage

Peptide ELISA

Detection limit 1:32000

Western Blot

0.1 - 0.3 ug/ml
Application Notes
WB: Approx. 50 kDa band observed in human colon, heart and ovary lysates (calculated MW of 50.2 kDa band according to NP_006320.2). An additional band of unknown identity was also consistently observed at 23 kDa. This band was successfully blocked by incubation with the immunizing peptide.
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA

Preservative

0.02% Sodium Azide

Concentration

0.5 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: Fibulin 5/DANCE

The protein encoded by this gene is a secreted, extracellular matrix protein containing an Arg-Gly-Asp (RGD) motif and calcium-binding EGF-like domains. It promotes adhesion of endothelial cells through interaction of integrins and the RGD motif. It is prominently expressed in developing arteries but less so in adult vessels. However, its expression is reinduced in balloon-injured vessels and atherosclerotic lesions, notably in intimal vascular smooth muscle cells and endothelial cells. Therefore, the protein encoded by this gene may play a role in vascular development and remodeling.

Alternate Names

DANCE, EVEC, FBLN5, UP50

Entrez Gene IDs

10516 (Human); 23876 (Mouse)

Gene Symbol

FBLN5

UniProt

NP_006320.2 (Human)

Additional Fibulin 5/DANCE Products

Product Documents for Fibulin 5/DANCE Antibody

Product Datasheets

Certificate of Analysis

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Product Specific Notices for Fibulin 5/DANCE Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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