FIH-1/HIF-1AN Antibody (12F11)
Novus Biologicals, part of Bio-Techne | Catalog # NBP3-26233
Recombinant monoclonal antibody expressed in HEK293F cells
Key Product Details
Species Reactivity
Human
Applications
ELISA, Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Western Blot
Label
Unconjugated
Antibody Source
Recombinant Monoclonal Rabbit IgG Clone # 12F11
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Product Specifications
Immunogen
A synthesized peptide derived from Human FIH-1/HIF-1AN [UniProt Q9NWT6]
Clonality
Monoclonal
Host
Rabbit
Isotype
IgG
Scientific Data Images for FIH-1/HIF-1AN Antibody (12F11)
Immunohistochemistry: FIH-1/HIF-1AN Antibody (12F11) [NBP3-26233] -
Immunohistochemistry: FIH-1/HIF-1AN Antibody (12F11) [NBP3-26233] - Image of FIH-1/HIF-1AN Antibody (12F11) diluted at 1:100 and staining in paraffin-embedded human testis tissue performed. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4C overnight. The primary is detected by a Goat anti-rabbit polymer IgG labeled by HRP and visualized using 0.05% DAB.
Immunohistochemistry: FIH-1/HIF-1AN Antibody (12F11) [NBP3-26233] -
Immunohistochemistry: FIH-1/HIF-1AN Antibody (12F11) [NBP3-26233] - Image of FIH-1/HIF-1AN Antibody (12F11) diluted at 1:100 and staining in paraffin-embedded human testis tissue performed. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit polymer IgG labeled by HRP and visualized using 0.05% DAB.
Western Blot: FIH-1/HIF-1AN Antibody (12F11) [NBP3-26233] -
Western Blot: FIH-1/HIF-1AN Antibody (12F11) [NBP3-26233] - Positive Western Blot detected in: Ntera-2 whole cell lysate, Hela whole cell lysate, A549 whole cell lysate, HepG2 whole cell lysate, PC3 whole cell lysate, U87 whole cell lysate.All lanes: FIH-1/HIF-1AN Antibody at 1: 2000
Secondary: Goat polyclonal to rabbit IgG at 1/50000 dilution.
Predicted band size: 41 kDa
Observed band size: 36-53 kDa
Applications for FIH-1/HIF-1AN Antibody (12F11)
Application
Recommended Usage
Flow Cytometry
1:50-1:200
Immunocytochemistry/ Immunofluorescence
1:50-1:200
Immunohistochemistry
1:50-1:200
Western Blot
1:500-1:2000
Formulation, Preparation, and Storage
Purification
Affinity purified
Formulation
PBS, pH 7.4, 150mM NaCl and 50% glycerol
Preservative
0.02% Sodium Azide
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at -20 to -70C. Avoid freeze-thaw cycles.
Background: FIH-1/HIF-1AN
Long Name
Factor Inhibiting HIF-1
Alternate Names
FIH1, HIF-1AN, HIF1AN, Peptide-aspartate beta-dioxygenase
Gene Symbol
HIF1AN
Additional FIH-1/HIF-1AN Products
Product Documents for FIH-1/HIF-1AN Antibody (12F11)
Product Specific Notices for FIH-1/HIF-1AN Antibody (12F11)
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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