FIH-1/HIF-1AN Antibody (9R7V4)

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-16256

Recombinant Monoclonal Antibody
Novus Biologicals, part of Bio-Techne
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Key Product Details

Validated by

Knockout/Knockdown

Species Reactivity

Human

Applications

Knockout Validated, Western Blot

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Clone # 9R7V4

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

View all FIH-1/HIF-1AN Antibodies »

Product Specifications

Immunogen

A synthetic peptide corresponding to a sequence within amino acids 1-100 of human FIH-1/HIF-1AN (Q9NWT6). MAATAAEAVASGSGEPREEAGALGPAWDESQLRSYSFPTRPIPRLSQSDPRAEELIENEEPVVLTDTNLVYPALKWDLEYLQENIGNGDFSVYSASTHKF

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for FIH-1/HIF-1AN Antibody (9R7V4)

Western Blot: FIH-1/HIF-1AN Antibody (9R7V4) [NBP3-16256]

Western Blot: FIH-1/HIF-1AN Antibody (9R7V4) [NBP3-16256]

Western Blot: FIH-1/HIF-1AN Antibody (9R7V4) [NBP3-16256] - Western blot analysis of extracts of U-87MG cells, using FIH-1/HIF-1AN Rabbit mAb (NBP3-16256) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 3min.
Knockout Validated: FIH-1/HIF-1AN Antibody (9R7V4) [NBP3-16256]

Knockout Validated: FIH-1/HIF-1AN Antibody (9R7V4) [NBP3-16256]

Knockout Validated: FIH-1/HIF-1AN Antibody (9R7V4) [NBP3-16256] - Western blot analysis of extracts from normal (control) and FIH-1/HIF-1AN knockout (KO) 293T cells, using FIH-1/HIF-1AN antibody (NBP3-16256) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 3min.

Applications for FIH-1/HIF-1AN Antibody (9R7V4)

Application
Recommended Usage

Western Blot

1:500 - 1:2000

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS, 0.05% BSA, 50% glycerol, pH7.3

Preservative

0.02% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: FIH-1/HIF-1AN

Factor Inhibiting HIF1 (FIH) protein facilitates repression of HIF-1 transcriptional activity by binding to VHL, which acts as a transcriptional corepressor. VHL inhibits HIF-1 alpha transactivation function by recruiting histone deacetylases. Involvement of VHL in association with FIH provides a unifying mechanism for the modulation of HIF-1 alpha protein stabilization and transcriptional activation in response to changes in cellular oxygen concentration.

Long Name

Factor Inhibiting HIF-1

Alternate Names

FIH1, HIF-1AN, HIF1AN, Peptide-aspartate beta-dioxygenase

Gene Symbol

HIF1AN

Additional FIH-1/HIF-1AN Products

Product Documents for FIH-1/HIF-1AN Antibody (9R7V4)

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for FIH-1/HIF-1AN Antibody (9R7V4)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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