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FIH-1/HIF-1AN Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-98999

Novus Biologicals, part of Bio-Techne
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NBP2-98999-100ul
NBP2-98999-50ul

Key Product Details

Species Reactivity

Validated:

Human

Predicted:

Mouse (100%). Backed by our 100% Guarantee.

Applications

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry-Paraffin

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Product Specifications

Immunogen

Produced in rabbits immunized with a synthetic peptide corresponding to the C-terminus of the Human FIH-1/HIF-1AN.

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Description

This antibody can be stored at 2C to 8C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20C to -80C. Avoid repeated freeze-thaw cycles.

Scientific Data Images for FIH-1/HIF-1AN Antibody

Immunocytochemistry/ Immunofluorescence: FIH-1/HIF-1AN Antibody [NBP2-98999]

Immunocytochemistry/ Immunofluorescence: FIH-1/HIF-1AN Antibody [NBP2-98999]

Immunocytochemistry/Immunofluorescence: FIH-1/HIF-1AN Antibody [NBP2-98999] - Immunofluorescence staining of FIH-1/HIF-1AN in HeLa cells. Cells were fixed with 4% PFA, blocked with 10% serum, and incubated with rabbit anti-human FIH-1/HIF-1AN polyclonal antibody (1:1000) at 4C overnight. Then cells were stained with the Alexa Fluor(R)488-conjugated Goat Anti-rabbit IgG Secondary antibody (green).Positive staining was localized to nucleus.
Immunohistochemistry-Paraffin: FIH-1/HIF-1AN Antibody [NBP2-98999]

Immunohistochemistry-Paraffin: FIH-1/HIF-1AN Antibody [NBP2-98999]

Immunohistochemistry-Paraffin: FIH-1/HIF-1AN Antibody [NBP2-98999] - Immunochemical staining of human FIH-1/HIF-1AN in human thyroid carcinoma with rabbit polyclonal antibody (1:300, formalin-fixed paraffin embedded sections).
Immunohistochemistry-Paraffin: FIH-1/HIF-1AN Antibody [NBP2-98999]

Immunohistochemistry-Paraffin: FIH-1/HIF-1AN Antibody [NBP2-98999]

Immunohistochemistry-Paraffin: FIH-1/HIF-1AN Antibody [NBP2-98999] - Immunochemical staining of human FIH-1/HIF-1AN in human kidney with rabbit polyclonal antibody (1:300, formalin-fixed paraffin embedded sections).

Applications for FIH-1/HIF-1AN Antibody

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:300-1:10000

Immunohistochemistry-Paraffin

1:100-1:500

Formulation, Preparation, and Storage

Purification

Antigen and protein A Affinity-purified

Formulation

0.2 um filtered solution in PBS

Preservative

No Preservative

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: FIH-1/HIF-1AN

Factor Inhibiting HIF1 (FIH) protein facilitates repression of HIF-1 transcriptional activity by binding to VHL, which acts as a transcriptional corepressor. VHL inhibits HIF-1 alpha transactivation function by recruiting histone deacetylases. Involvement of VHL in association with FIH provides a unifying mechanism for the modulation of HIF-1 alpha protein stabilization and transcriptional activation in response to changes in cellular oxygen concentration.

Long Name

Factor Inhibiting HIF-1

Alternate Names

FIH1, HIF-1AN, HIF1AN, Peptide-aspartate beta-dioxygenase

Gene Symbol

HIF1AN

Additional FIH-1/HIF-1AN Products

Product Documents for FIH-1/HIF-1AN Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for FIH-1/HIF-1AN Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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