Filaggrin Antibody (FLG/1561) - Azide and BSA Free
Novus Biologicals, part of Bio-Techne | Catalog # NBP2-54366
Conjugate
Catalog #
Key Product Details
Species Reactivity
Human
Applications
Immunohistochemistry, Immunohistochemistry-Paraffin, Protein Array
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG1 kappa Clone # FLG/1561
Format
Azide and BSA Free
Concentration
1 mg/ml
Product Specifications
Immunogen
Recombinant human Filaggrin protein fragment (aa 198-288) (exact sequence is proprietary) (Uniprot: P20930)
Localization
Cytoplasmic
Marker
Keratinocyte Differentiation Marker
Clonality
Monoclonal
Host
Mouse
Isotype
IgG1 kappa
Description
1.0 mg/ml of antibody purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS WITHOUT BSA & azide. Also available at 200 ug/ml WITH BSA & azide (NBP2-53243).
Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80C.
Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80C.
Scientific Data Images for Filaggrin Antibody (FLG/1561) - Azide and BSA Free
Immunohistochemistry-Paraffin: Filaggrin Antibody (FLG/1561) - Azide and BSA Free [NBP2-54366]
Immunohistochemistry-Paraffin: Filaggrin Antibody (FLG/1561) - Azide and BSA Free [NBP2-54366] - Formalin-fixed, paraffin-embedded human Skin stained with Filaggrin Mouse Monoclonal Antibody (FLG/1561).Protein Array: Filaggrin Antibody (FLG/1561) - Azide and BSA Free [NBP2-54366]
Protein Array: Filaggrin Antibody (FLG/1561) - Azide and BSA Free [NBP2-54366] - Analysis of Protein Array containing >19,000 full-length human proteins using Filaggrin Antibody (FLG/1561) Z- and S- Score: The Z-score represents the strength of a signal that a monoclonal antibody (Monoclonal Antibody) (in combination with a fluorescently-tagged anti-IgG secondary antibody) produces when binding to a particular protein on the HuProt(TM) array. Z-scores are described in units of standard deviations (SDs) above the mean value of all signals generated on that array. If targets on HuProt(TM) are arranged in descending order of the Z-score, the S-score is the difference (also in units of SDs) between the Z-score. S-score therefore represents the relative target specificity of a Monoclonal Antibody to its intended target. A Monoclonal Antibody is considered to specific to its intended target, if the Monoclonal Antibody has an S-score of at least 2.5.Applications for Filaggrin Antibody (FLG/1561) - Azide and BSA Free
Application
Recommended Usage
Immunohistochemistry
Optimal dilutions of this antibody should be experimentally determined.
Immunohistochemistry-Paraffin
Optimal dilutions of this antibody should be experimentally determined.
Protein Array
Optimal dilutions of this antibody should be experimentally determined.
Application Notes
Use in Western Blot reported in scientific literature (PMID: 32102205).
Immunohistochemistry (Formalin-fixed): 1-2ug/ml for 30 minutes at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95C followed by cooling at RT for 20 minutes.
Optimal dilution for a specific application should be determined.
Immunohistochemistry (Formalin-fixed): 1-2ug/ml for 30 minutes at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95C followed by cooling at RT for 20 minutes.
Optimal dilution for a specific application should be determined.
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Protein A or G purified
Formulation
10 mM PBS
Format
Azide and BSA Free
Preservative
No Preservative
Concentration
1 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at -20 to -80C. Avoid freeze-thaw cycles.
Background: Filaggrin
Alternate Names
ATOD2, epidermal filaggrin, filaggrin, Profilaggrin
Gene Symbol
FLG
Additional Filaggrin Products
Product Documents for Filaggrin Antibody (FLG/1561) - Azide and BSA Free
Product Specific Notices for Filaggrin Antibody (FLG/1561) - Azide and BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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