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Key Product Details

Species Reactivity

Human

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Protein Array

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 kappa Clone # FLG/1561

Format

Azide and BSA Free

Concentration

1 mg/ml

Product Specifications

Immunogen

Recombinant human Filaggrin protein fragment (aa 198-288) (exact sequence is proprietary) (Uniprot: P20930)

Localization

Cytoplasmic

Marker

Keratinocyte Differentiation Marker

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1 kappa

Description

1.0 mg/ml of antibody purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS WITHOUT BSA & azide. Also available at 200 ug/ml WITH BSA & azide (NBP2-53243).

Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80C.

Scientific Data Images for Filaggrin Antibody (FLG/1561) - Azide and BSA Free

Immunohistochemistry-Paraffin: Filaggrin Antibody (FLG/1561) - Azide and BSA Free [NBP2-54366]

Immunohistochemistry-Paraffin: Filaggrin Antibody (FLG/1561) - Azide and BSA Free [NBP2-54366]

Immunohistochemistry-Paraffin: Filaggrin Antibody (FLG/1561) - Azide and BSA Free [NBP2-54366] - Formalin-fixed, paraffin-embedded human Skin stained with Filaggrin Mouse Monoclonal Antibody (FLG/1561).
Protein Array: Filaggrin Antibody (FLG/1561) - Azide and BSA Free [NBP2-54366]

Protein Array: Filaggrin Antibody (FLG/1561) - Azide and BSA Free [NBP2-54366]

Protein Array: Filaggrin Antibody (FLG/1561) - Azide and BSA Free [NBP2-54366] - Analysis of Protein Array containing >19,000 full-length human proteins using Filaggrin Antibody (FLG/1561) Z- and S- Score: The Z-score represents the strength of a signal that a monoclonal antibody (Monoclonal Antibody) (in combination with a fluorescently-tagged anti-IgG secondary antibody) produces when binding to a particular protein on the HuProt(TM) array. Z-scores are described in units of standard deviations (SDs) above the mean value of all signals generated on that array. If targets on HuProt(TM) are arranged in descending order of the Z-score, the S-score is the difference (also in units of SDs) between the Z-score. S-score therefore represents the relative target specificity of a Monoclonal Antibody to its intended target. A Monoclonal Antibody is considered to specific to its intended target, if the Monoclonal Antibody has an S-score of at least 2.5.

Applications for Filaggrin Antibody (FLG/1561) - Azide and BSA Free

Application
Recommended Usage

Immunohistochemistry

Optimal dilutions of this antibody should be experimentally determined.

Immunohistochemistry-Paraffin

Optimal dilutions of this antibody should be experimentally determined.

Protein Array

Optimal dilutions of this antibody should be experimentally determined.
Application Notes
Use in Western Blot reported in scientific literature (PMID: 32102205).
Immunohistochemistry (Formalin-fixed): 1-2ug/ml for 30 minutes at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95C followed by cooling at RT for 20 minutes.
Optimal dilution for a specific application should be determined.
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Protein A or G purified

Formulation

10 mM PBS

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20 to -80C. Avoid freeze-thaw cycles.

Background: Filaggrin

Alternate Names

ATOD2, epidermal filaggrin, filaggrin, Profilaggrin

Gene Symbol

FLG

Additional Filaggrin Products

Product Documents for Filaggrin Antibody (FLG/1561) - Azide and BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for Filaggrin Antibody (FLG/1561) - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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