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Key Product Details

Species Reactivity

Validated:

Human

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 kappa Clone # FLG/1562

Format

Azide and BSA Free

Concentration

1 mg/ml

Product Summary for Filaggrin Antibody (FLG/1562) - Azide and BSA Free

Immunogen

Recombinant human Filaggrin protein fragment (aa 198-288) (exact sequence is proprietary) (Uniprot: P20930)

Localization

Cytoplasmic

Marker

Keratinocyte Differentiation Marker

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1 kappa

Description

1.0 mg/ml of antibody purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS WITHOUT BSA & azide. Also available at 200 ug/ml WITH BSA & azide (NBP2-53245).

Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80C.

Scientific Data Images for Filaggrin Antibody (FLG/1562) - Azide and BSA Free

Immunohistochemistry-Paraffin: Filaggrin Antibody (FLG/1562) - Azide and BSA Free [NBP2-54362]

Immunohistochemistry-Paraffin: Filaggrin Antibody (FLG/1562) - Azide and BSA Free [NBP2-54362]

Immunohistochemistry-Paraffin: Filaggrin Antibody (FLG/1562) [NBP2-54362] - Formalin-fixed, paraffin-embedded human Skin stained with Filaggrin Monoclonal Antibody (FLG/1562).

Applications for Filaggrin Antibody (FLG/1562) - Azide and BSA Free

Application
Recommended Usage

Immunohistochemistry

Optimal dilutions of this antibody should be experimentally determined.

Immunohistochemistry-Paraffin

Optimal dilutions of this antibody should be experimentally determined.
Application Notes
Immunohistochemistry (Formalin-fixed): 1-2ug/ml for 30 minutes at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95C followed by cooling at RT for 20 minutes.
Optimal dilution for a specific application should be determined.
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Protein A or G purified

Formulation

10 mM PBS

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20 to -80C. Avoid freeze-thaw cycles.

Background: Filaggrin

Alternate Names

ATOD2, epidermal filaggrin, filaggrin, Profilaggrin

Gene Symbol

FLG

Additional Filaggrin Products

Product Documents for Filaggrin Antibody (FLG/1562) - Azide and BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for Filaggrin Antibody (FLG/1562) - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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