Key Product Details

Species Reactivity

Validated:

Rat, Non-species specific

Cited:

Rat

Applications

Validated:

ELISA, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Cited:

ELISA

Label

Unconjugated

Antibody Source

Polyclonal Goat IgG

Format

BSA Free

Concentration

1.0 mg/ml

View all FITC Antibodies »

Product Specifications

Immunogen

FITC conjugated to KLH.

Reactivity Notes

Rat reactivity reported in scientific literature (PMID:32940130)

Specificity

This reacts specifically with FITC. There is no reaction with KLH.

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Applications for FITC Antibody - BSA Free

Application
Recommended Usage

ELISA

1:1000 - 1:30000

Immunocytochemistry/ Immunofluorescence

1:200-1:2000

Immunohistochemistry

1:200-1:2000

Immunohistochemistry-Paraffin

1:200- 1:2000

Western Blot

1:1000 - 1:30000
Application Notes
Elisa-for coating plates 1:100 - 1:500.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS

Format

BSA Free

Preservative

0.09% Sodium Azide

Concentration

1.0 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C. Do not freeze.

Background: FITC

FITC (fluorescein isothiocyanate) is a fluorochrome dye that absorbs ultraviolet or blue light causing molecules to become excited and emit a visible yellow-green light. This emission ceases upon removal of the light causing the excitation. Fluorochrome labeling provides rapid, accurate localization of antigen-antibody interaction when one of the reactants is part of a cell, tissue or other biological structure. FITC is a commonly used marker for antibodies in immunofluorescent techniques since the conjugation of FITC to proteins is relatively easy and does not, in general, destroy the biological activity of the labeled protein. FITC is widely used as a hapten to label different proteins.

Long Name

Fluorescein Isothiocyanate

Alternate Names

Fluorescein Isothiocyanate

Additional FITC Products

Product Documents for FITC Antibody - BSA Free

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Download SDS

Product Specific Notices for FITC Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Privacy and Cookie Policy
Site Map
© Copyright 2024 Bio-Techne. All Rights Reserved.