Key Product Details

Validated by

Biological Validation

Species Reactivity

Validated:

Human

Applications

Immunoprecipitation, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

1.0 mg/ml

View all FKBP15 Antibodies »

Product Summary for FKBP15 Antibody

Immunogen

A synthetic peptide mapping to a region between residues 960 and 1020 of human FK506-binding protein 135 using the numbering given in TrEMBL entry Q9Y4D0 (GeneID 23307).

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for FKBP15 Antibody

Western Blot: FKBP15 Antibody [NB100-423]

Western Blot: FKBP15 Antibody [NB100-423]

Western Blot: FKBP15 Antibody [NB100-423] - Detection of Human FKPB-135 by Western Blot. Samples: Whole cell lysate (50 ug) from HeLa and 293T cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-FKPB-135 antibody NB100-423 used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.
Western Blot: FKBP15 Antibody [NB100-423]

Western Blot: FKBP15 Antibody [NB100-423]

Western Blot: FKBP15 Antibody [NB100-423] - Detection of human FKBP-135 in Western Blot and immunoprecipitation, using NB100-423. Samples: HeLa nuclear extracts. Exposure times of (A) 25 minutes and (B) 10 minutes.
FKBP15 Antibody [NB100-423] - Detection of human FKPB-135 by western blot of immunoprecipitates. Samples: Whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) from HeLa cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-FKPB-135 antibody NB100-423 used for IP at 6 ug per reaction. FKPB-135 was also immunoprecipitated by rabbit anti-FKPB-135 antibody NB100-252. For blotting immunoprecipitated FKPB-135, NB100-423 was used at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.

Applications for FKBP15 Antibody

Application
Recommended Usage

Immunoprecipitation

2 - 10 ug/mg of lysate

Western Blot

1:2000-1:10000
Application Notes
Western Blot and immunoprecipitation. Immunohistochemistry has not been tested. *The investigator should determine the optimal working dilution for a specific application.
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

Tris-Citrate/Phosphate (pH 7.0 - 8.0)

Preservative

0.09% Sodium Azide

Concentration

1.0 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C. Do not freeze.

Background: FKBP15

Immunophilins are a family of soluble receptors capable of binding to one of two major immunosuppressant agents; cyclosporin A (CsA) or FK506. Proteins that bind FK506 are termed FK506 Binding Proteins (FKBPs) and those that bind cyclosporin A are called cyclophilins (CyP). Immunophilins function as cis-trans peptidyl-prolyl isomerases (PPIase) whose activity is inhibited by their respective immunosuppressant compounds. Thus, immunophilins accelerate folding of some proteins both in vivo and in vitro by catalyzing slow steps in the initial folding and rearrangement of proline-containing proteins.

Alternate Names

FK506 binding protein 15, 133kDa, FK506-binding protein 133kDa, FK506-binding protein 15, FKBP133, FKBP-133, FKBP-15,133 kDa FKBP, PPP1R76,133 kDa FK506-binding protein, regulatory subunit 76, WAFL

Entrez Gene IDs

23307 (Human)

Gene Symbol

FKBP15

UniProt

Q9Y4D0 (Human)

Additional FKBP15 Products

Product Documents for FKBP15 Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Download SDS

Product Specific Notices for FKBP15 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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