FLAP Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NB300-891

Novus Biologicals, part of Bio-Techne
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NB300-891
NB300-891-0.025mg
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Key Product Details

Species Reactivity

Validated:

Human, Mouse, Rat

Cited:

Human, Mouse, Rat

Applications

Validated:

Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Peptide ELISA

Cited:

IF/IHC, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Frozen, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Goat IgG

Concentration

0.5 mg/ml

View all Flap Antibodies »

Product Specifications

Immunogen

Peptide with sequence C-KTISTTISPLLLIP corresponding to C-Terminus according to NP_001620.2.

Reactivity Notes

Mouse reactivity reported in scientific literature (PMID: 27198524). Rat reactivity reported in scientific literature (PMID: 30735559).

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Scientific Data Images for FLAP Antibody

Immunocytochemistry/ Immunofluorescence: FLAP Antibody [NB300-891]

Immunocytochemistry/ Immunofluorescence: FLAP Antibody [NB300-891]

Immunocytochemistry/Immunofluorescence: FLAP Antibody [NB300-891] - Immunofluorescence analysis of paraformaldehyde fixed MCF7 cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml), showing strong endplasmic reticulum staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml).
Immunohistochemistry-Paraffin: FLAP Antibody [NB300-891]

Immunohistochemistry-Paraffin: FLAP Antibody [NB300-891]

Immunohistochemistry-Paraffin: FLAP Antibody [NB300-891] - Negative Control showing staining of paraffin embedded Human Spleen, with no primary antibody.
Flow Cytometry: FLAP Antibody [NB300-891]

Flow Cytometry: FLAP Antibody [NB300-891]

Flow Cytometry: FLAP Antibody [NB300-891] - Flow cytometric analysis of paraformaldehyde fixed MCF7 cells (blue line), permeabilized with 0.5% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (1ug/ml). IgG control: Unimmunized goat IgG (black line) followed by Alexa Fluor 488 secondary antibody.
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Applications for FLAP Antibody

Application
Recommended Usage

Flow Cytometry

10 ug/mL

Immunohistochemistry-Paraffin

6 ug/mL

Peptide ELISA

Detection limit 1:6000
Application Notes
Use in ICC/IF reported in scientific literature (PMID: 30735559). Please note that the current batch of this antibody is not validated for use in WB. Publications citing use in WB were using previous batches.
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA

Preservative

0.02% Sodium Azide

Concentration

0.5 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: Flap

This gene encodes a protein which, with 5-lipoxygenase, is required for leukotriene synthesis. Leukotrienes are arachidonic acid metabolites which have been implicated in various types of inflammatory responses, including asthma, arthritis and psoriasis. This protein localizes to the plasma membrane. Inhibitors of its function impede translocation of 5-lipoxygenase from the cytoplasm to the cell membrane and inhibit 5-lipoxygenase activation.

Alternate Names

arachidonate 5-lipoxygenase-activating protein, FLAPMK-886-binding protein

Entrez Gene IDs

241 (Human)

Gene Symbol

ALOX5AP

UniProt

NP_001620. (Human)

Additional Flap Products

Product Documents for FLAP Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for FLAP Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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