FLI1 Antibody (1F6)

Novus Biologicals, part of Bio-Techne | Catalog # H00002313-M05

Novus Biologicals, part of Bio-Techne
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Key Product Details

Species Reactivity

Human

Applications

ELISA, Immunocytochemistry/ Immunofluorescence, Knockdown Validated, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2b Kappa Clone # 1F6

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

View all FLI1 Antibodies »

Product Specifications

Immunogen

FLI1 (AAH01670, 1 a.a. ~ 452 a.a) full-length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. MDGTIKEALSVVSDGQSLFDSAYGAAAHLPKADMAASGSPDYGQPHKINPLPPQQEWISQPVRVNVKREYDHMNGSRESPVDCSVSKCSKLVGGGESNPMNYNSYMDEKNGPPPPNMTTNERRVIVPADPTLWTQEHVRQWLEWAIKEYSLMEIDTSFFQNMDGKELCKMNKEDFLRATTLYNTEVLLSHLSYLRESSLLAYNTTSHTDQSSRLSVKEDPSYDSVRRGAWGNNMNSGLNKSPPLGGAQTISKNTEQRPQPDPYQILGPTSSRLANPGSGQIQLWQFLLELLSDSANASCITWEGTNGEFKMTDPDEVARRWGERISKPNMNYDKLSRALRYYYDKNIMTKVHGKRYAYKFDFHGIAQALQPHPTESSMYKYPSDISYMPSYHAHQQKVNFVPPHPSSMPVTSSSFFGAASQYWTSPTGGIYPNPNVPRHPNTHVPSHLGSYY

Specificity

FLI1 (1F6)

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2b Kappa

Description

Quality control test: Antibody Reactive Against Recombinant Protein.

Scientific Data Images for FLI1 Antibody (1F6)

Western Blot: FLI1 Antibody (1F6) [H00002313-M05]

Western Blot: FLI1 Antibody (1F6) [H00002313-M05]

Western Blot: FLI1 Antibody (1F6) [H00002313-M05] - Analysis of FLI1 expression in transfected 293T cell line by FLI1 monoclonal antibody (M05), clone 1F6. Lane 1: FLI1 transfected lysatE (50.982 KDa). Lane 2: Non-transfected lysate.
Immunocytochemistry/ Immunofluorescence: FLI1 Antibody (1F6) [H00002313-M05]

Immunocytochemistry/ Immunofluorescence: FLI1 Antibody (1F6) [H00002313-M05]

Immunocytochemistry/Immunofluorescence: FLI1 Antibody (1F6) [H00002313-M05] - Analysis of monoclonal antibody to FLI1 on HeLa cell. Antibody concentration 10 ug/ml
Western Blot: FLI1 Antibody (1F6) [H00002313-M05]

Western Blot: FLI1 Antibody (1F6) [H00002313-M05]

Western Blot: FLI1 Antibody (1F6) [H00002313-M05] - Western blot analysis of FLI1 over-expressed 293 cell line, cotransfected with FLI1 Validated Chimera RNAi or non-transfected control. Blot probed with H00002313-M05. GAPDH (36.1 kDa) used as loading control.

Applications for FLI1 Antibody (1F6)

Application
Recommended Usage

Western Blot

1:500
Application Notes
Antibody reactivity against transfected lysate and recombinant protein for WB. It has been used for ELISA and RNAi Validation.
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

IgG purified

Formulation

In 1x PBS, pH 7.4

Preservative

No Preservative

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: FLI1

Ets-1 is the prototype member of a family of genes identified on the basis of homology to the v-Ets oncogene isolated from the E26 erythroblastosis virus. This family of genes currently includes Ets-1, Ets-2 Erg-1, Erg-2, Elk, E74, Fli-1, PU.1 and PEA3. Members of the Ets gene family exhibit varied pattern of tissue expression, and share a highly conserved carboxy terminal domain containing a sequence related to the SV40 large T antigen nuclear localization signal sequence.

Long Name

Friend Leukemia Integration 1

Alternate Names

ERGB, SIC-1

Entrez Gene IDs

2313 (Human)

Gene Symbol

FLI1

OMIM

193067 (Human)

UniProt

AAH01670 (Human)

Additional FLI1 Products

Product Documents for FLI1 Antibody (1F6)

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Download SDS

Product Specific Notices for FLI1 Antibody (1F6)

This product is produced by and distributed for Abnova, a company based in Taiwan.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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