Flightless I Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-87925

Novus Biologicals, part of Bio-Techne
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NBP1-87925
NBP1-87925-25ul
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Key Product Details

Species Reactivity

Validated:

Human, Mouse

Cited:

Mouse

Predicted:

Rat (95%). Backed by our 100% Guarantee.

Applications

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

View all Flightless I Antibodies »

Product Specifications

Immunogen

This antibody was developed against Recombinant Protein corresponding to amino acids: YILDCWSDVFIWLGRKSPRLVRAAALKLGQELCGMLHRPRHATVSRSLEGTEAQVFKAKFKNWDDVLTVDYTRNAEAVLQSPGLSGKVKRDAEKKDQMKADLTALFLPRQPPMSLAEAEQLMEEWNEDL

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for Flightless I Antibody

Western Blot: Flightless I Antibody [NBP1-87925]

Western Blot: Flightless I Antibody [NBP1-87925]

Western Blot: Flightless I Antibody [NBP1-87925] - Lane 1: Marker [kDa] 230, 130, 95, 72, 56, 36, 28, 17, 11. Lane 2: Human cell line RT-4. Lane 3: Human cell line U-251MG sp. Lane 4: Human plasma (IgG/HSA depleted). Lane 5: Human liver tissue
Immunocytochemistry/ Immunofluorescence: Flightless I Antibody [NBP1-87925]

Immunocytochemistry/ Immunofluorescence: Flightless I Antibody [NBP1-87925]

Immunocytochemistry/Immunofluorescence: Flightless I Antibody [NBP1-87925] - Staining of human cell line U-2 OS shows localization to nucleoplasm, cytosol & microtubule organizing center.
Immunohistochemistry-Paraffin: Flightless I Antibody [NBP1-87925]

Immunohistochemistry-Paraffin: Flightless I Antibody [NBP1-87925]

Immunohistochemistry-Paraffin: Flightless I Antibody [NBP1-87925] - Staining of human testis shows moderate cytoplasmic positivity in Leydig cells.
View 5 more images

Applications for Flightless I Antibody

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

0.25-2 ug/ml

Immunohistochemistry

1:50 - 1:200

Immunohistochemistry-Paraffin

1:50 - 1:200

Western Blot

0.04-0.4 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. Immunocytochemistry/Immunofluorescence Fixation Permeabilization: Use PFA/Triton X-100.
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Flightless I

Alternate Names

FLI, Fli1, flightless I homolog (Drosophila), FLILflightless I (Drosophila) homolog, MGC39265, protein flightless-1 homolog

Gene Symbol

FLII

Additional Flightless I Products

Product Documents for Flightless I Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Download SDS

Product Specific Notices for Flightless I Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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