FLIP Antibody (PSH01-95)

Western Blot: FLIP Antibody (PSH01-95) [NBP3-32342]

Western Blot: FLIP Antibody (PSH01-95) [NBP3-32342] - Western blot analysis of FLIP on different lysates with Rabbit anti-FLIP antibody (NBP3-32342) at 1/1,000 dilution.

Lane 1: A549 cell lysate
Lane 2: HeLa cell lysate
Lane 3: 293T cell lysate
Lane 4: K-562 cell lysate
Lane 5: HCT 116 cell lysate
Lane 6: MDA-MB-468 cell lysate
Lane 7: MDA-MB-231 cell lysate
Lane 8: Jurkat cell lysate
Lane 9: SW480 cell lysate

Lysates/proteins at 20 ug/Lane.

Predicted band size: 55 kDa
Observed band size: 55/25 kDa

Exposure time: 5 minutes;

4-20% SDS-PAGE gel.

Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32342) at 1/1,000 dilution was used in 5% NFDM/TBST at 4C overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.