FLIP Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NB100-56141

Novus Biologicals, part of Bio-Techne
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NB100-56141
NB100-56141SS
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Key Product Details

Species Reactivity

Validated:

Human

Applications

Immunohistochemistry, Immunohistochemistry-Frozen, Immunohistochemistry-Paraffin, Immunoprecipitation, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

This product is unpurified. The exact concentration of antibody is not quantifiable.

View all FLIP Antibodies »

Product Summary for FLIP Antibody

Immunogen

A synthetic peptide corresponding to amino acids 75-92 (DRKAVETHLLRNPHLVS) of human FLIP was used as immunogen, GenBank no. gi|12643547. This amino acid sequnce is 100% conserved in FLIP long and short, and various FLIP isoforms as denoted in the NCBI BLAST data base. http://www.ncbi.nlm.nih.gov/blast/.

Specificity

The gene encoding FLIP produces, by alternative splicing, 24 different transcripts, all with introns, putatively encoding 22 different protein isoforms.

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for FLIP Antibody

Western Blot: FLIP Antibody [NB100-56141]

Western Blot: FLIP Antibody [NB100-56141]

Western Blot: FLIP Antibody [NB100-56141] - analysis of FLIP in human ovarian cancer cell lines using anti-FLIP antibody. The primary antibody was used at a dilution of 1:200 and incubated for overnight at 4C in 5% milk in PBST. Image from verified customer review.
Immunohistochemistry: FLIP Antibody [NB100-56141]

Immunohistochemistry: FLIP Antibody [NB100-56141]

Immunohistochemistry: FLIP Antibody [NB100-56141] - Immunhohistochemical analysis of FLIP in tissues cores from a formalin-fixed, paraffin-embedded human brain tumor microarray using NB100-56141 at 1:2000. A-C, tissue cores from a patient with gemistocytoma. A, normal adjacent brain tissue. Astrocytes are positive for Flip expression. B, gemistocytoma (Grade II). Gemistocytes are positive for Flip expression. C, peritumoural oedma brain tissue. Reactive astrocytocytes are positive for FLIP expression. C1, a higher magnification of C. Collectively, the data shows that astrocytes (normal), reactive astrocytes and gemistocytes are positive for Flip expression. Hematoxylin-eosin counterstain.
Immunohistochemistry: FLIP Antibody [NB100-56141]

Immunohistochemistry: FLIP Antibody [NB100-56141]

Immunohistochemistry: FLIP Antibody [NB100-56141] - Immunhohistochemical analysis of FLIP in two tissues cores from a formalin-fixed, paraffin-embedded human brain tumor microarray using NB100-56141 at 1:2000. A, gemistocytoma (Grade II) positive for FLIP expression. B, gemistocytoma (Grade II) negative for FLIP expression. A1 and A2 are higher magnifications from A. A, A1, and A2 show expression of FLIP in the gemistocytes of the tumor. Hematoxylin-eosin counterstain.

Applications for FLIP Antibody

Application
Recommended Usage

Immunohistochemistry-Paraffin

1:1000-1:5000

Immunoprecipitation

1:50-1:200

Western Blot

1:1000-1:2000
Application Notes
IHC-Frozen: Users should optimize according to model and immunodetection system used (secondary reagents). The molecular weights of these isoforms may vary. Therefore, the molecular weight of FLIP observed by western blot may vary according to the isoforms present in the sample.
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Reviewed Applications

Read 1 review rated 4 using NB100-56141 in the following applications:

Formulation, Preparation, and Storage

Purification

Unpurified

Formulation

Whole antisera

Preservative

0.05% Sodium Azide

Concentration

This product is unpurified. The exact concentration of antibody is not quantifiable.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: FLIP

Cell death signals are transduced by death domain (DD)- containing adapter molecules and members of the caspase (ICE/CED-3) protease family. Most if not all, forms of apoptosis involve activation of the caspases. Caspases-8 (FLICE) and -10 (FLICE2) are two pivotal members in the caspase family. FLICE-inhibitory proteins were identified in virus and human and designated v-FLIPs and FLIP, respectively. The human FLIP was also cloned by several labs independently and termed Casper , I-FLICE, FLAME-1, CASH and CLARP3-7. FLIP contains two death effector domains (DEDs) and a caspase-like domain. FLIP interacts with adapter protein FADD and caspase-8 and 10, and potently inhibits apoptosis induced by death receptors (reviewed in Peter, 2004). However, the functions of FLIP remain to be fully elucidated and there is some evidence FLIP may also act as an activator of caspase-8/-10 in certain cases, thereby taking on an apoptosis promoting role. FLIP has a short form and a long form. Additionally, four splice variants of c-FLIPs have been identified and termed FLIP a, b, g, and d. NB100-56141 recognizes FLIP. Human FLIP long is a 480 amino acid protein, GenBank no. gi|12643547. Human FLIP short is a 221 amino acid protein, GenBank no. AAC51623.1. NB100-56141 will recognize FLIP long and short, and FLIP isoforms that contain the peptide immunogen sequence (DRKAVETHLLRNPHLVSD), including FLIP a, b, g, and d.

Long Name

FLICE Inhibitory Proteins

Alternate Names

CASPER, CFLAR, FLAME-1, I-FLICE

Gene Symbol

CFLAR

Additional FLIP Products

Product Documents for FLIP Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for FLIP Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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