Flotillin-1 Antibody (8I2S7)
Novus Biologicals, part of Bio-Techne | Catalog # NBP3-16152
Recombinant Monoclonal Antibody
Key Product Details
Species Reactivity
Human, Mouse, Rat
Applications
ELISA, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot
Label
Unconjugated
Antibody Source
Recombinant Monoclonal Rabbit IgG Clone # 8I2S7
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Product Specifications
Immunogen
A synthetic peptide corresponding to a sequence within amino acids 328-427 of human Flotillin-1 (O75955). GARARAEAEQMAKKAEAFQLYQEAAQLDMLLEKLPQVAEEISGPLTSANKITLVSSGSGTMGAAKVTGEVLDILTRLPESVERLTGVSISQVNHKPLRTA
Clonality
Monoclonal
Host
Rabbit
Isotype
IgG
Theoretical MW
47 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Scientific Data Images for Flotillin-1 Antibody (8I2S7)
![Western Blot: Flotillin-1 Antibody (8I2S7) [NBP3-16152]](https://resources.bio-techne.com/images/products/Flotillin-1-Antibody-8I2S7-Western-Blot-NBP3-16152-img0004.jpg "Western Blot: Flotillin-1 Antibody (8I2S7) [NBP3-16152]")
Western Blot: Flotillin-1 Antibody (8I2S7) [NBP3-16152]
Western Blot: Flotillin-1 Antibody (8I2S7) [NBP3-16152] - Analysis of extracts of various cell lines, using Flotillin-1 Rabbit mAb (NBP3-16152) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.![Western Blot: Flotillin-1 Antibody (8I2S7) [NBP3-16152]](https://resources.bio-techne.com/images/products/Flotillin-1-Antibody-8I2S7-Western-Blot-NBP3-16152-img0005.jpg "Western Blot: Flotillin-1 Antibody (8I2S7) [NBP3-16152]")
Western Blot: Flotillin-1 Antibody (8I2S7) [NBP3-16152]
Western Blot: Flotillin-1 Antibody (8I2S7) [NBP3-16152] - Analysis of extracts of various cell lines, using Flotillin-1 Rabbit mAb (NBP3-16152) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 3min.
Immunohistochemistry: Flotillin-1 Antibody (8I2S7) [NBP3-16152] -
Immunohistochemistry: Flotillin-1 Antibody (8I2S7) [NBP3-16152] - Immunohistochemistry analysis of paraffin-embedded Human liver cancer tissue using Flotillin-1 Rabbit mAb at a dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.
Applications for Flotillin-1 Antibody (8I2S7)
Application
Recommended Usage
Immunohistochemistry
1:50 - 1:200
Western Blot
1:500 - 1:2000
Formulation, Preparation, and Storage
Purification
Affinity purified
Formulation
PBS (pH 7.3), 50% glycerol, 0.05% BSA
Preservative
0.02% Sodium Azide
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at -20C. Avoid freeze-thaw cycles.
Background: Flotillin-1
Alternate Names
flotillin 1, flotillin-1, integral membrane component of caveolae
Gene Symbol
FLOT1
Additional Flotillin-1 Products
Product Documents for Flotillin-1 Antibody (8I2S7)
Product Specific Notices for Flotillin-1 Antibody (8I2S7)
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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