Flotillin-1 Antibody
Novus Biologicals, part of Bio-Techne | Catalog # NBP1-87498
![Western Blot: Flotillin-1 Antibody [NBP1-87498]](https://resources.bio-techne.com/images/products/Flotillin-1-Antibody-Western-Blot-NBP1-87498-img0012.jpg "Western Blot: Flotillin-1 Antibody [NBP1-87498]")
![Immunohistochemistry-Paraffin: Flotillin-1 Antibody [NBP1-87498]](https://resources.bio-techne.com/images/products/Flotillin-1-Antibody-Immunohistochemistry-NBP1-87498-img0013.jpg "Immunohistochemistry-Paraffin: Flotillin-1 Antibody [NBP1-87498]")
Key Product Details
Validated by
Orthogonal Validation
Species Reactivity
Validated:
Human, Mouse, Rat
Cited:
Human
Applications
Validated:
Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot
Cited:
Immunocytochemistry/ Immunofluorescence
Label
Unconjugated
Antibody Source
Polyclonal Rabbit IgG
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Product Specifications
Immunogen
This antibody was developed against Recombinant Protein corresponding to amino acids: HQRAIMAHMTVEEIYKDRQKFSEQVFKVASSDLVNMGISVVSYTLKDIHDDQDYLHSLGKARTAQVQKDARIGEAEAKRDAGIREAKAKQEKVSAQYLSEIEMAKAQRDYELKKAAYDIEVNTRRAQADLAYQLQVAKTKQQIEEQR
Clonality
Polyclonal
Host
Rabbit
Isotype
IgG
Scientific Data Images for Flotillin-1 Antibody
![Western Blot: Flotillin-1 Antibody [NBP1-87498]](https://resources.bio-techne.com/images/products/Flotillin-1-Antibody-Western-Blot-NBP1-87498-img0009.jpg "Western Blot: Flotillin-1 Antibody [NBP1-87498]")
Western Blot: Flotillin-1 Antibody [NBP1-87498]
Western Blot: Flotillin-1 Antibody [NBP1-87498] - Analysis in mouse cell line NIH-3T3 and rat cell line NBT-II.
Applications for Flotillin-1 Antibody
Application
Recommended Usage
Immunocytochemistry/ Immunofluorescence
Reported in scientific literature (PMID:32180897)
Immunohistochemistry
1:500 - 1:1000
Immunohistochemistry-Paraffin
1:500 - 1:1000
Western Blot
0.04-0.4 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended.
Formulation, Preparation, and Storage
Purification
Immunogen affinity purified
Formulation
PBS (pH 7.2) and 40% Glycerol
Preservative
0.02% Sodium Azide
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Background: Flotillin-1
Alternate Names
flotillin 1, flotillin-1, integral membrane component of caveolae
Gene Symbol
FLOT1
Additional Flotillin-1 Products
Product Documents for Flotillin-1 Antibody
Product Specific Notices for Flotillin-1 Antibody
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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