FMRP [p Ser499] Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-29509

Novus Biologicals, part of Bio-Techne
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NBP2-29509
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Key Product Details

Validated by

Biological Validation

Species Reactivity

Validated:

Human, Mouse, Rat, Chicken

Predicted:

Bovine (100%), Canine (100%), Chicken (100%), Human (100%), Primate (100%), Sheep (100%), Xenopus (100%), Zebrafish (100%). Backed by our 100% Guarantee.

Applications

Immunohistochemistry, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

View all FMRP Antibodies »

Product Specifications

Immunogen

Synthetic phospho-peptide corresponding to amino acid residues surrounding Ser499 conjugated to KLH. Accession # Q80WE1

Modification

p Ser499

Specificity

Specific for endogenous levels of the ~71 kDa FMRP protein phosphorylated at Ser499. Immunolabeling is completely eliminated by treatment with lambda-phosphatase.

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

71 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for FMRP [p Ser499] Antibody

Western Blot: FMRP [p Ser499] Antibody [NBP2-29509]

Western Blot: FMRP [p Ser499] Antibody [NBP2-29509]

Western Blot: FMRP [p Ser499] Antibody [NBP2-29509] - Western blot of rat hippocampal lysate showing specific immunolabeling of the ~71k FMRP protein phosphorylated at Ser499 (Control). The phosphospecificity of this labeling is shown in the second lane. The blot is identical to the control except that the lysate was incubated in lambda-Ptase (400 units/100ul lysate for 30 min) before being exposed to the FMRP Ser499 antibody. The immunolabeling is completely eliminated by treatment with lambda-Ptase.

Applications for FMRP [p Ser499] Antibody

Application
Recommended Usage

Immunohistochemistry

1:250 - 1:1000

Western Blot

1:1000

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Formulation

10mM HEPES (pH 7.5), 0.15M NaCl, 0.1 mg/ml BSA and 50% Glycerol

Preservative

No Preservative

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: FMRP

Fragile X Mental Retardation Protein (FMRP) is an RNA-binding protein that plays an essential role in cognitive brain function. Mutations in the FMR1 gene, which codes for FMRP, can result in fragile X syndrome, autism, as well as other cognitive deficits (Brown et al.,1998, Goodlin-Jones et al., 2004). Phosphorylation of the highly conserved Ser499 has been shown to trigger hierarchical phosphorylation of nearby serines and may play a role in suppressing target mRNA translation (Ceman et al., 2003, Narayanan et al. 2008).

Alternate Names

FMRPFRAXAPOF, fragile X mental retardation 1, fragile X mental retardation 1 protein, MGC87458, POF1, premature ovarian failure 1, Protein FMR-1

Entrez Gene IDs

24948 (Rat)

Gene Symbol

FMR1

UniProt

Q80WE1 (Rat)

Additional FMRP Products

Product Documents for FMRP [p Ser499] Antibody

Product Datasheets

Certificate of Analysis

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Product Specific Notices for FMRP [p Ser499] Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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