Key Product Details

Validated by

Independent Antibodies, Biological Validation

Species Reactivity

Human

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Immunoprecipitation, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

1.0 mg/ml

View all FNBP1L Antibodies »

Product Specifications

Immunogen

The immunogen this antibody was made to, maps to a region between residue 450 and 500 of human Transducer of Cdc42-Dependent Actin Assembly Protein 1 using the numbering given in entry NP_001157945.1 (GeneID 54874).

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for FNBP1L Antibody

Western Blot: FNBP1L Antibody [NBP1-80569]

Western Blot: FNBP1L Antibody [NBP1-80569]

Western Blot: FNBP1L Antibody [NBP1-80569] - Whole cell lysate from Jurkat (15 and 50 mcg for WB; 1 mg for IP, 20% of IP loaded), 293T (T; 50 mcg) and HeLa (H; 50 mcg) cells. Antibodies: Affinity purified rabbit anti-Toca-1 antibody used for WB at 0.1 mcg/ml (A) and 1 mcg/ml (B) and used for IP at 6 mcg/mg lysate. Toca-1 was also immunoprecipitated by rabbit anti-Toca-1 antibody NBP1-80563 which recognizes an upstream epitope.
Immunohistochemistry-Paraffin: FNBP1L Antibody [NBP1-80569]

Immunohistochemistry-Paraffin: FNBP1L Antibody [NBP1-80569]

Immunohistochemistry-Paraffin: FNBP1L Antibody [NBP1-80569] - Section of human lung carcinoma. Antibody: Affinity purified rabbit anti-Toca-1used at a dilution of 1:1,000 (1ug/ml) Detection: DAB

Applications for FNBP1L Antibody

Application
Recommended Usage

Immunohistochemistry

1:500 - 1:2000

Immunohistochemistry-Paraffin

1:500-1:2000

Immunoprecipitation

2-10 ug/mg

Western Blot

1:2000-1:10000
Application Notes
Epitope retrieval with citrate buffer pH6.0 is recommended for FFPE tissue sections.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

Tris-Citrate/Phosphate (pH 7.0 - 8.0)

Preservative

0.09% Sodium Azide

Concentration

1.0 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C. Do not freeze.

Background: FNBP1L

The protein encoded by the FNBP1L gene binds to both CDC42 and N-WASP. This protein promotes CDC42-induced actin polymerization by activating the N-WASP-WIP complex and, therefore, is involved in a pathway that links cell surface signals to the actin cytoskeleton. Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq]

Alternate Names

C1orf39, chromosome 1 open reading frame 39, FLJ20275, formin binding protein 1-like, formin-binding protein 1-like, toca-1, TOCA1Transducer of Cdc42-dependent actin assembly protein 1, transducer of Cdc42-dependent actin assembly 1

Gene Symbol

FNBP1L

UniProt

NP_001157945.1 (Human)

Additional FNBP1L Products

Product Documents for FNBP1L Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for FNBP1L Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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