Follistatin Antibody (FST/4281)
Novus Biologicals, part of Bio-Techne | Catalog # NBP3-07491
![Immunohistochemistry-Paraffin: Follistatin Antibody (FST/4281) [NBP3-07491]](https://resources.bio-techne.com/images/products/Follistatin-Antibody-FST-4281-Immunohistochemistry-Paraffin-NBP3-07491-img0001.jpg "Immunohistochemistry-Paraffin: Follistatin Antibody (FST/4281) [NBP3-07491]")
Conjugate
Catalog #
Forumulation
Catalog #
Key Product Details
Species Reactivity
Validated:
Human
Applications
ELISA, Immunohistochemistry-Paraffin
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG1 Clone # FST/4281
Concentration
0.2 mg/ml
Product Summary for Follistatin Antibody (FST/4281)
Immunogen
Raised to human Follistatin (Fst) 288 and recognizes both human Fst 288 and 315 (Uniprot: P19883)
Localization
Secreted.
Specificity
Follistatin is a single-chain glycosylated protein that inhibits follicle stimulating hormone (FSH) release. Alternative splicing of Follistatin mRNA yields two isoforms, FS315 and FS288. FS288 is the main cell-surface form and binds to surface heparin sulphate proteoglycans. FST/4281 is a clone raised against recombinant Fst 288, and is used in combination, commonly as the capture, with antibody FST/4282 in a two site ELISA for the detection of Follistatin.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG1
Theoretical MW
37 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Description
200ug/ml of antibody purified from Bioreactor Concentrate by Protein A or G. Prepared in 10 mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0 mg/ml. (NBP3-08813)
Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80 C.
Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80 C.
Scientific Data Images for Follistatin Antibody (FST/4281)
Immunohistochemistry-Paraffin: Follistatin Antibody (FST/4281) [NBP3-07491]
Immunohistochemistry-Paraffin: Follistatin Antibody (FST/4281) [NBP3-07491] - Formalin-fixed, paraffin-embedded human testis stained with Follistatin Mouse Monoclonal Antibody (FST/4281).Applications for Follistatin Antibody (FST/4281)
Application
Recommended Usage
Immunohistochemistry-Paraffin
1-2 ug/ml
Application Notes
ELISA: Use in combination with clone FST/4282 for two-site ELISA.
Immunohistochemistry (Formalin-fixed): 1-2ug/ml for 30 minutes at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95C followed by cooling at RT for 20 minutes.
Optimal dilution for a specific application should be determined.
Immunohistochemistry (Formalin-fixed): 1-2ug/ml for 30 minutes at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95C followed by cooling at RT for 20 minutes.
Optimal dilution for a specific application should be determined.
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Protein A or G purified
Formulation
10 mM PBS with 0.05% BSA
Preservative
0.05% Sodium Azide
Concentration
0.2 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C.
Background: Follistatin
Additional Follistatin Products
Product Documents for Follistatin Antibody (FST/4281)
Product Specific Notices for Follistatin Antibody (FST/4281)
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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