Follistatin Antibody (FST/4282)
Novus Biologicals, part of Bio-Techne | Catalog # NBP3-07492
Conjugate
Catalog #
Forumulation
Catalog #
Key Product Details
Species Reactivity
Validated:
Human
Applications
ELISA, Immunocytochemistry/ Immunofluorescence, Western Blot
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG1 Clone # FST/4282
Concentration
0.2 mg/ml
Product Summary for Follistatin Antibody (FST/4282)
Immunogen
Recognizes human Follistatin isoform FS288, raised against recombinant human FS288. (Uniprot: P19883)
Localization
Secreted.
Specificity
Follistatin is a single-chain glycosylated protein that inhibits follicle stimulating hormone (FSH) release. Alternative splicing of Follistatin mRNA yields two isoforms, FS315 and FS288. FS288 is the main cell-surface form and binds to surface heparin sulphate proteoglycans. FST/4282 recognizes recombinant human Follistatin 288, allowing for detection of FSH levels using various analysis methods. This antibody also works in a two site ELISA with FST/4281.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG1
Theoretical MW
37 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Description
200ug/ml of antibody purified from Bioreactor Concentrate by Protein A or G. Prepared in 10 mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0 mg/ml. (NBP3-08814)
Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80 C.
Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80 C.
Applications for Follistatin Antibody (FST/4282)
Application
Recommended Usage
ELISA
1 ug/ml
Immunocytochemistry/ Immunofluorescence
70 ug/ml
Western Blot
1-2 ug/ml
Application Notes
Optimal dilution for a specific application should be determined.
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Protein A or G purified
Formulation
10 mM PBS with 0.05% BSA
Preservative
0.05% Sodium Azide
Concentration
0.2 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C.
Background: Follistatin
Additional Follistatin Products
Product Documents for Follistatin Antibody (FST/4282)
Product Specific Notices for Follistatin Antibody (FST/4282)
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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