FOXI1 Antibody (PCRP-FOXL1-1F8)
Novus Biologicals, part of Bio-Techne | Catalog # NBP3-13886
Conjugate
Catalog #
Forumulation
Catalog #
Key Product Details
Species Reactivity
Validated:
Human
Applications
Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry-Paraffin, Immunoprecipitation, Protein Array, Western Blot
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG1 Clone # PCRP-FOXL1-1F8
Concentration
0.2 mg/ml
Product Summary for FOXI1 Antibody (PCRP-FOXL1-1F8)
Immunogen
Recombinant fragment (around aa104-258) of human FOXI1 protein (exact sequence is proprietary) (Uniprot: Q12951)
Reactivity Notes
Predicted to react in Rat.
Localization
Nucleus
Clonality
Monoclonal
Host
Mouse
Isotype
IgG1
Description
200ug/ml of antibody purified from Bioreactor Concentrate by Protein A or G. Prepared in 10 mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0 mg/ml. (NBP3-14224)
Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80 C. Non-hazardous.
Theoretical molecular weight: 36-41kDa
Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80 C. Non-hazardous.
Theoretical molecular weight: 36-41kDa
Scientific Data Images for FOXI1 Antibody (PCRP-FOXL1-1F8)
Immunocytochemistry/ Immunofluorescence: FOXI1 Antibody (PCRP-FOXL1-1F8) [NBP3-13886]
Immunocytochemistry/Immunofluorescence: FOXI1 Antibody (PCRP-FOXL1-1F8) [NBP3-13886] - Immunofluorescence analysis of PFA-fixed HeLa cells. FOXI1 Antibody (PCRP-FOXL1-1F8) followed by goat anti-mouse IgG-CF488 (green). CF640A phalloidin (red).Immunohistochemistry-Paraffin: FOXI1 Antibody (PCRP-FOXL1-1F8) [NBP3-13886]
Immunohistochemistry-Paraffin: FOXI1 Antibody (PCRP-FOXL1-1F8) [NBP3-13886] - Formalin-fixed, paraffin-embedded human prostate stained. FOXI1 antibody (PCRP-FOXL1-1F8) at 2ug/ml. HIER: Tris/EDTA, pH9.0, 45min. HRP-polymer, 30min. DAB, 5min.Flow Cytometry: FOXI1 Antibody (PCRP-FOXL1-1F8) [NBP3-13886]
Flow Cytometry: FOXI1 Antibody (PCRP-FOXL1-1F8) [NBP3-13886] - Flow cytometric analysis of PFA-fixed HeLa cells. FOXI1 antibody (PCRP-FOXL1-1F8) followed by goat anti-mouse IgG-CF488 (blue), unstained cells (red).Applications for FOXI1 Antibody (PCRP-FOXL1-1F8)
Application
Recommended Usage
Flow Cytometry
1-2 ug/million cells
Immunocytochemistry/ Immunofluorescence
1-2 ug/ml
Immunohistochemistry-Paraffin
1-2 ug/ml
Immunoprecipitation
1-2 ug/100-500 ug protein
Western Blot
1-2 ug/ml
Application Notes
Immunohistochemistry (Formalin-fixed): 1-2ug/ml for 30 minutes at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95C followed by cooling at RT for 20 minutes
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Protein A or G purified
Formulation
10 mM PBS with 0.05% BSA
Preservative
0.05% Sodium Azide
Concentration
0.2 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C.
Background: FOXI1
Alternate Names
FKHL10HFH3, forkhead box I1, forkhead box protein I1, forkhead-like 10, forkhead-related activator 6, Forkhead-related protein FKHL10, Forkhead-related transcription factor 6, FREAC-6, FREAC6FKH10, Hepatocyte nuclear factor 3 forkhead homolog 3, HFH-3, HNF-3/fork-head homolog 3, HNF-3/fork-head homolog-3, MGC34197
Gene Symbol
FOXI1
UniProt
Additional FOXI1 Products
Product Documents for FOXI1 Antibody (PCRP-FOXL1-1F8)
Product Specific Notices for FOXI1 Antibody (PCRP-FOXL1-1F8)
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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