FoxJ1/HFH4 Antibody (CL3989)

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-59032

Novus Biologicals, part of Bio-Techne
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NBP2-59032
NBP2-59032-25ul
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Key Product Details

Validated by

Orthogonal Validation

Species Reactivity

Validated:

Human

Predicted:

Mouse (91%). Backed by our 100% Guarantee.

Applications

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # CL3989

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

View all FoxJ1 Antibodies »

Product Specifications

Immunogen

This antibody was developed against a recombinant protein corresponding to the following amino acid sequence:PREKDEPGKGGFWRIDPQYAERLLSGAFKKRRLPPVHIHPAFARQAAQEPSAVPRAGPLTVNTEAQQLLREFEEATGEAGWGAGEGRLGHKRKQPLPKRVAKVPRPPSTLLPTPEEQGELEPLKG

Epitope

EPGKGGFWRI

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any questions

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Scientific Data Images for FoxJ1/HFH4 Antibody (CL3989)

Immunohistochemistry-Paraffin: FoxJ1/HFH4 Antibody (CL3989) [NBP2-59032]

Immunohistochemistry-Paraffin: FoxJ1/HFH4 Antibody (CL3989) [NBP2-59032]

Immunohistochemistry-Paraffin: FoxJ1/HFH4 Antibody (CL3989) [NBP2-59032] - Analysis in human fallopian tube and skeletal muscle tissues using NBP2-59032 antibody. Corresponding FOXJ1 RNA-seq data are presented for the same tissues.
Immunocytochemistry/ Immunofluorescence: FoxJ1/HFH4 Antibody (CL3989) [NBP2-59032]

Immunocytochemistry/ Immunofluorescence: FoxJ1/HFH4 Antibody (CL3989) [NBP2-59032]

Immunocytochemistry/Immunofluorescence: FoxJ1/HFH4 Antibody (CL3989) [NBP2-59032] - Staining of BEWO cells using the Anti-FOXJ1 monoclonal antibody, showing specific staining in the nucleoplasm in green. Microtubule- and nuclear probes are visualized in red and blue, respectively (where available).
Immunohistochemistry-Paraffin: FoxJ1/HFH4 Antibody (CL3989) [NBP2-59032]

Immunohistochemistry-Paraffin: FoxJ1/HFH4 Antibody (CL3989) [NBP2-59032]

Immunohistochemistry-Paraffin: FoxJ1/HFH4 Antibody (CL3989) [NBP2-59032] - Staining of human tonsil shows no positivity in germinal center cells as expected.
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Applications for FoxJ1/HFH4 Antibody (CL3989)

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

2-10 ug/ml

Immunohistochemistry

1:1000 - 1:2000

Immunohistochemistry-Paraffin

1:1000 - 1:2000
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. For ICC/IF, PFA/Triton X-100 is recommended for fixation/permeabilization.

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: FoxJ1

FOXJ1 is a member of the forkhead gene family, which was originally identified in Drosophila. The forkhead family is composed of transcription factors with a conserved 100-amino acid DNA-binding motif.[supplied by OMIM]

Long Name

Forkhead Box J1

Alternate Names

FKHL13, HFH-4

Gene Symbol

FOXJ1

Additional FoxJ1 Products

Product Documents for FoxJ1/HFH4 Antibody (CL3989)

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for FoxJ1/HFH4 Antibody (CL3989)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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